Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,014,976	C→T	A28T (GCT→ACT)	yqeC ←	uncharacterized protein YqeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,014,976	0	C	T	100.0%	113.0 / NA	33	A28T (GCT→ACT)	yqeC	uncharacterized protein YqeC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/14);  total (19/14)

TCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTAT  >  NC_000913/3014898‑3015048                                                                               |                                                                         tcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTAc                                                                        <  1:493934/81‑1 (MQ=255)  cgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTAc                                                                        >  2:493978/1‑80 (MQ=255)   gtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTAcc                                                                       <  1:493935/80‑1 (MQ=255)       tGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACcaacaa                                                                  >  2:493970/1‑81 (MQ=255)               acacGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAAt                                                           >  2:493984/1‑80 (MQ=255)               acacGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAAt                                                           >  2:493974/1‑80 (MQ=255)               acacGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAAt                                                           >  1:493982/1‑80 (MQ=255)                       gCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTgg                                                   >  2:493983/1‑80 (MQ=255)                       gCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTgg                                                   >  1:493991/1‑80 (MQ=255)                         cGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGAc                                                <  1:493937/81‑1 (MQ=255)                         cGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGAc                                                <  2:493935/81‑1 (MQ=255)                           cTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGt                                              >  1:493988/1‑81 (MQ=255)                             aGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGttt                                            >  2:493975/1‑81 (MQ=255)                             aGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGttt                                            >  1:493986/1‑81 (MQ=255)                                     gCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCAc                                    <  2:493937/81‑1 (MQ=255)                                     gCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCAc                                    <  1:493939/81‑1 (MQ=255)                                       aGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCt                                  <  1:493940/81‑1 (MQ=255)                                         ttCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTTGGACGTTTCTGCGCACCTa                                 >  2:493977/1‑80 (MQ=255)                                          tCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTaaa                               >  2:493993/1‑81 (MQ=255)                                           cTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAAt                              >  2:493976/1‑81 (MQ=255)                                            tGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAAt                              >  2:493973/1‑80 (MQ=255)                                              ccagccaAAAAAGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCaa                           >  2:493979/1‑81 (MQ=255)                                                    aaaaaaGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACt                      >  1:493981/1‑80 (MQ=255)                                                      aaaaGCAAGCTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAAt                   >  2:493982/1‑81 (MQ=255)                                                         agcaagcTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGcc                <  1:493941/81‑1 (MQ=255)                                                         agcaagcTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGcc                >  1:493967/1‑81 (MQ=255)                                                          gcaagcTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCg               <  2:493941/81‑1 (MQ=255)                                                              gcTGGTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATgg           <  2:493942/81‑1 (MQ=255)                                                                  gTTTTCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTc        <  1:493944/80‑1 (MQ=255)                                                                     ttCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAAc     <  1:493945/80‑1 (MQ=255)                                                                      tCCCCCCAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACt    <  1:493946/80‑1 (MQ=255)                                                                       ccccccAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACta   >  2:493988/1‑80 (MQ=255)                                                                        cccccAGTACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACtat  <  2:493946/80‑1 (MQ=255)                                                                               |                                                                         TCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTAT  >  NC_000913/3014898‑3015048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑