Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,028,232	C→A	A371D (GCT→GAT)	ghxQ →	guanine/hypoxanthine transporter GhxQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,028,232	0	C	A	100.0%	97.0 / NA	28	A371D (GCT→GAT)	ghxQ	guanine/hypoxanthine transporter GhxQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/10);  total (18/10)

TAGTGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGCTCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATG  >  NC_000913/3028154‑3028305                                                                               |                                                                          tAGTGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGatc                                                                         <  1:496726/81‑1 (MQ=255)  aGTGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGatct                                                                        >  2:496762/1‑81 (MQ=255)    tGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGa                                                                      >  1:496764/1‑81 (MQ=255)     gggggCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGAt                                                                     >  1:496760/1‑81 (MQ=255)      ggggCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGAt                                                                     <  1:496727/80‑1 (MQ=255)         gCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTAc                                                                 <  2:496727/81‑1 (MQ=255)              aTTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAgg                                                            >  2:496765/1‑81 (MQ=255)               ttCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGt                                                           >  1:496759/1‑81 (MQ=255)               ttCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGt                                                           >  1:496756/1‑81 (MQ=255)                  cTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGttt                                                         <  1:496729/80‑1 (MQ=255)                  cTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGttt                                                         <  2:496729/80‑1 (MQ=255)                       aaTTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGAt                                                   >  2:496756/1‑81 (MQ=255)                        aTTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATg                                                  >  1:496761/1‑81 (MQ=255)                        aTTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATg                                                  <  2:496730/81‑1 (MQ=255)                         ttCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGt                                                 <  1:496732/81‑1 (MQ=255)                           cTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGtt                                                >  2:496753/1‑80 (MQ=255)                                  tATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACg                                        >  1:496782/1‑81 (MQ=255)                                  tATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACg                                        >  1:496767/1‑81 (MQ=255)                                          cTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAg                                <  2:496732/81‑1 (MQ=255)                                             tCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCt                              <  2:496733/80‑1 (MQ=255)                                               aTTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGa                           >  2:496768/1‑81 (MQ=255)                                                          cTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGAtt                >  1:496779/1‑81 (MQ=255)                                                             gTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGAtttt              <  2:496734/80‑1 (MQ=255)                                                             gTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTa             >  1:496768/1‑81 (MQ=255)                                                               tACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTAtt           >  1:496770/1‑81 (MQ=255)                                                               tACGCCACTGCACCCGATCTGATGTACGCAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTAtt           >  2:496770/1‑81 (MQ=255)                                                                aCGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTAtt           >  1:496765/1‑80 (MQ=255)                                                                    cACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGc      >  1:496773/1‑81 (MQ=255)                                                                    cACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGc      <  2:496735/81‑1 (MQ=255)                                                                        gCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTAtg  >  2:496789/1‑81 (MQ=255)                                                                               |                                                                          TAGTGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGCTCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATG  >  NC_000913/3028154‑3028305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑