Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,030,090	A→G	V281A (GTC→GCC)	ygfT ←	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,030,090	0	A	G	100.0%	96.0 / NA	29	V281A (GTC→GCC)	ygfT	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/16);  total (13/16)

ACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAAT  >  NC_000913/3030012‑3030163                                                                               |                                                                          acaacTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCt                                                                         >  1:497133/1‑81 (MQ=255)  caacTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTg                                                                        >  2:497131/1‑81 (MQ=255)   aacTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTgc                                                                       <  1:497098/81‑1 (MQ=255)   aacTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTgc                                                                       <  2:497098/81‑1 (MQ=255)      tttGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGcc                                                                    >  1:497139/1‑81 (MQ=255)      tttGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGcc                                                                    >  1:497144/1‑81 (MQ=255)        tGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGccc                                                                   <  2:497100/80‑1 (MQ=255)        tGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGccc                                                                   <  2:497099/80‑1 (MQ=255)          cTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGa                                                                 <  2:497101/80‑1 (MQ=255)                   ggACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCttt                                                        >  1:497152/1‑80 (MQ=255)                      cGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCaaa                                                    <  1:497103/81‑1 (MQ=255)                          aGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTAc                                                <  2:497103/81‑1 (MQ=255)                              cATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATgc                                            <  2:497104/81‑1 (MQ=255)                                tCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATgcgc                                          <  2:497105/81‑1 (MQ=255)                                 cGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGcc                                         >  1:497138/1‑81 (MQ=255)                                  gCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCt                                        <  1:497107/81‑1 (MQ=255)                                   ccAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCtt                                       >  2:497142/1‑81 (MQ=255)                                    cAGCGCGGTATCGGTGATGTAGCGTTCCAGAATACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTc                                      <  2:497107/81‑1 (MQ=255)                                            tATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAAAc                              >  2:497150/1‑81 (MQ=255)                                                 gTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTcc                         >  1:497141/1‑81 (MQ=255)                                                 gTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTcc                         <  2:497108/81‑1 (MQ=255)                                                  tGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCt                        <  1:497110/81‑1 (MQ=255)                                                     tGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAATACATGCGCCTTCACAAAGACGGTCCtgtg                     <  2:497110/81‑1 (MQ=255)                                                           gTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATa               >  1:497148/1‑81 (MQ=255)                                                            ttCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATAc              >  2:497145/1‑81 (MQ=255)                                                            ttCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATAc              >  2:497146/1‑81 (MQ=255)                                                                   ttACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCg       >  2:497160/1‑81 (MQ=255)                                                                       cGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCaaa   <  2:497111/81‑1 (MQ=255)                                                                        gATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAAt  <  2:497112/81‑1 (MQ=255)                                                                               |                                                                          ACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAAT  >  NC_000913/3030012‑3030163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑