Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,064,651	A→G	H220R (CAC→CGC)	scpB →	methylmalonyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,064,651	0	A	G	100.0%	135.2 / NA	39	H220R (CAC→CGC)	scpB	methylmalonyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/25);  total (14/25)

CACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGG  >  NC_000913/3064578‑3064725                                                                          |                                                                           caccacATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCAt                                                                      >  1:504728/1‑80 (MQ=255) caccacATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCAt                                                                      >  2:504732/1‑80 (MQ=255)    cacaTCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAAc                                                                  >  2:504733/1‑81 (MQ=255)     acaTCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGCGCTGCGTGTACTGGGCGAAGCACGCACCATGAACt                                                                 <  2:504678/81‑1 (MQ=255)       aTCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTcc                                                               <  1:504680/81‑1 (MQ=255)             gagaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGaa                                                         >  2:504738/1‑81 (MQ=255)              agaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATg                                                           <  1:504681/78‑1 (MQ=255)              agaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGa                                                          <  1:504682/79‑1 (MQ=255)               gaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAAtt                                                       <  2:504682/81‑1 (MQ=255)                aaaGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAAttt                                                      <  2:504683/81‑1 (MQ=255)                aaaGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAAttt                                                      >  1:504725/1‑81 (MQ=255)                  aGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGa                                                    <  1:504685/81‑1 (MQ=255)                      ccGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGt                                                <  2:504685/81‑1 (MQ=255)                             ccATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAgg                                         <  2:504687/81‑1 (MQ=255)                             ccATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAgg                                         <  1:504687/81‑1 (MQ=255)                              cATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAggg                                        <  1:504689/81‑1 (MQ=255)                              cATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAggg                                        >  1:504735/1‑81 (MQ=255)                                  gCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATg                                    >  1:504749/1‑81 (MQ=255)                                   ccGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATgc                                   <  2:504691/81‑1 (MQ=255)                                   ccGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATgc                                   <  1:504691/81‑1 (MQ=255)                                   ccGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATg                                    <  2:504689/80‑1 (MQ=255)                                      ttATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGcgcc                                >  2:504731/1‑81 (MQ=255)                                      ttATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGcgcc                                <  1:504693/81‑1 (MQ=255)                                        aTCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGcgccg                               <  2:504693/80‑1 (MQ=255)                                              gaagaGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGgtg                        <  2:504694/81‑1 (MQ=255)                                               aagaGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGgtg                        >  2:504735/1‑80 (MQ=255)                                                 gagCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTAt                     <  1:504696/81‑1 (MQ=255)                                                    cTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGa                   >  2:504745/1‑80 (MQ=255)                                                       cGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGc               <  2:504696/81‑1 (MQ=255)                                                       cGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGc               <  1:504698/81‑1 (MQ=255)                                                          gtACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCg              >  1:504738/1‑79 (MQ=255)                                                          gtACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCg              >  2:504744/1‑79 (MQ=255)                                                             cTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGAt         <  1:504699/81‑1 (MQ=255)                                                               gggCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTa       >  1:504726/1‑81 (MQ=255)                                                                ggCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTa       <  1:504700/80‑1 (MQ=255)                                                                 gCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTAcc     <  1:504701/81‑1 (MQ=255)                                                                  cGAAGAACGCACAATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCa    <  1:504702/81‑1 (MQ=255)                                                                    aaGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGATGATTACCAg   >  2:504739/1‑80 (MQ=255)                                                                    aaGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAgg  <  1:504703/81‑1 (MQ=255)                                                                          |                                                                           CACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGG  >  NC_000913/3064578‑3064725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑