Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,875	C→T	A329T (GCT→ACT)	cmtA ←	mannitol‑specific PTS enzyme IICB component CmtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,875	0	C	T	100.0%	140.6 / NA	39	A329T (GCT→ACT)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/18);  total (21/18)

CATTGGCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGT  >  NC_000913/3077797‑3077949                                                                               |                                                                           cATTGGCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTaa                                                                          <  1:507485/81‑1 (MQ=255)    tGGCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAg                                                                        >  1:507530/1‑80 (MQ=255)     ggCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAgg                                                                       <  2:507485/80‑1 (MQ=255)          actgaGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGacac                                                                   >  1:507531/1‑79 (MQ=255)           ctgaGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAg                                                                <  1:507487/81‑1 (MQ=255)            tgaGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAgg                                                               >  1:507551/1‑81 (MQ=255)                 aaaCTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGtacc                                                          >  2:507534/1‑81 (MQ=255)                 aaaCTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGtacc                                                          <  1:507488/81‑1 (MQ=255)                 aaaCTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGtacc                                                          >  2:507542/1‑81 (MQ=255)                 aaaCTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGtacc                                                          >  1:507553/1‑81 (MQ=255)                          ttCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTaa                                                  >  1:507540/1‑80 (MQ=255)                            cGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAaca                                                <  1:507489/80‑1 (MQ=255)                                ctcCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCgg                                            >  2:507536/1‑80 (MQ=255)                                  ccGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCaa                                         >  2:507557/1‑81 (MQ=255)                                    gTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAAtt                                       >  1:507545/1‑81 (MQ=255)                                      ttCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGt                                     >  1:507554/1‑81 (MQ=255)                                         cACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGc                                  <  2:507489/81‑1 (MQ=255)                                          aCCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGcc                                 <  2:507490/81‑1 (MQ=255)                                            cGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAg                               <  1:507494/81‑1 (MQ=255)                                            cGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAg                               <  1:507492/81‑1 (MQ=255)                                            cGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAg                               <  1:507493/81‑1 (MQ=255)                                             gTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAgg                              <  2:507494/81‑1 (MQ=255)                                               tttttCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGaa                            >  1:507548/1‑81 (MQ=255)                                                 tttCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAAc                           >  1:507539/1‑80 (MQ=255)                                                  ttCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACg                          <  2:507495/80‑1 (MQ=255)                                                    ccATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAg                        <  2:507497/80‑1 (MQ=255)                                                    ccATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAg                        <  1:507497/80‑1 (MQ=255)                                                      aTCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCt                     <  1:507499/81‑1 (MQ=255)                                                           cAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCgg                >  2:507540/1‑81 (MQ=255)                                                              taacAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGt             <  2:507499/78‑1 (MQ=255)                                                               aTCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGt             >  1:507561/1‑80 (MQ=255)                                                                tCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGt             >  1:507574/1‑79 (MQ=255)                                                                  aGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGt         <  2:507500/81‑1 (MQ=255)                                                                   gCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTg        >  2:507564/1‑81 (MQ=255)                                                                     gAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGc       >  1:507552/1‑80 (MQ=255)                                                                      aaGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCa     <  2:507501/81‑1 (MQ=255)                                                                       aGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAg    >  2:507537/1‑81 (MQ=255)                                                                       aGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAg    >  2:507566/1‑81 (MQ=255)                                                                          aaTAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAgat  >  2:507543/1‑78 (MQ=255)                                                                               |                                                                           CATTGGCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGT  >  NC_000913/3077797‑3077949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑