Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,304	C→T	A168T (GCC→ACC)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,304	0	C	T	100.0%	153.7 / NA	43	A168T (GCC→ACC)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/16);  total (27/16)

CCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATA  >  NC_000913/3123226‑3123381                                                                               |                                                                              ccaccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGa                                                                             >  2:517243/1‑81 (MQ=255)  caccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGAt                                                                            <  2:517223/81‑1 (MQ=255)   accTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGAt                                                                            >  2:517251/1‑80 (MQ=255)    ccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATaa                                                                          <  1:517225/81‑1 (MQ=255)     cTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAAc                                                                         >  1:517256/1‑81 (MQ=255)        aaaCGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTg                                                                      >  2:517270/1‑81 (MQ=255)           cGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCt                                                                    >  2:517266/1‑80 (MQ=255)            gCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCa                                                                  >  1:517254/1‑81 (MQ=255)                ccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACcgc                                                              >  1:517277/1‑81 (MQ=255)                ccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACcgc                                                              >  1:517292/1‑81 (MQ=255)                   caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGtt                                                           >  2:517268/1‑81 (MQ=255)                     tCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGc                                                         <  2:517225/81‑1 (MQ=255)                       cTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCgg                                                       <  2:517226/81‑1 (MQ=255)                       cTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCg                                                        >  1:517279/1‑80 (MQ=255)                          aTAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATc                                                    <  2:517227/81‑1 (MQ=255)                           tAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCg                                                   >  1:517295/1‑81 (MQ=255)                                 ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGcc                                              <  1:517229/80‑1 (MQ=255)                                    ttttCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTg                                          <  2:517229/81‑1 (MQ=255)                                        ccAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGAcc                                       >  2:517265/1‑80 (MQ=255)                                         cAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAt                                     >  1:517293/1‑81 (MQ=255)                                         cAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAt                                     <  1:517231/81‑1 (MQ=255)                                          aGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATc                                    <  2:517232/81‑1 (MQ=255)                                          aGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATc                                    <  1:517232/81‑1 (MQ=255)                                             ggTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGcc                                 >  1:517275/1‑81 (MQ=255)                                              gTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGccc                                <  1:517234/81‑1 (MQ=255)                                                 gagaTTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCt                              <  2:517234/80‑1 (MQ=255)                                                    aTTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCt                          >  2:517281/1‑81 (MQ=255)                                                    aTTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCt                          >  2:517262/1‑81 (MQ=255)                                                      tCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGc                        >  1:517278/1‑81 (MQ=255)                                                       cATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCg                       >  2:517284/1‑81 (MQ=255)                                                        aTCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCgg                      <  1:517236/81‑1 (MQ=255)                                                         tCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCgg                      >  1:517272/1‑80 (MQ=255)                                                          cGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGga                    >  1:517294/1‑81 (MQ=255)                                                           gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat                   >  2:517286/1‑81 (MQ=255)                                                           gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat                   >  2:517289/1‑81 (MQ=255)                                                                aaCGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGt              >  2:517273/1‑81 (MQ=255)                                                                 aCGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTc             <  2:517236/81‑1 (MQ=255)                                                                      gAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGc        >  2:517269/1‑81 (MQ=255)                                                                      gAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGc        >  2:517282/1‑81 (MQ=255)                                                                       aaCCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGcc       <  2:517237/81‑1 (MQ=255)                                                                            aGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtata  <  2:517238/81‑1 (MQ=255)                                                                            aGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtat   >  1:517284/1‑80 (MQ=255)                                                                            aGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtat   >  1:517289/1‑80 (MQ=255)                                                                               |                                                                              CCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATA  >  NC_000913/3123226‑3123381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑