Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,124,738	G→A	A241V (GCC→GTC)	glcF ←	glycolate dehydrogenase, putative iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,124,738	0	G	A	100.0%	106.9 / NA	30	A241V (GCC→GTC)	glcF	glycolate dehydrogenase, putative iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/19);  total (11/19)

CATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATT  >  NC_000913/3124664‑3124811                                                                           |                                                                          cATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTg                                                                     <  1:517542/81‑1 (MQ=255)        tttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAAt                                                              >  1:517578/1‑81 (MQ=255)         ttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAAt                                                              <  1:517543/80‑1 (MQ=255)             gCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCgg                                                         <  1:517544/81‑1 (MQ=255)              cATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCggg                                                        >  1:517571/1‑81 (MQ=255)               aTCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGc                                                       >  1:517582/1‑81 (MQ=255)                tCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGc                                                       >  1:517586/1‑80 (MQ=255)                    cccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGccacc                                                   <  1:517548/80‑1 (MQ=255)                    cccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGccacc                                                   <  1:517545/80‑1 (MQ=255)                    cccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGccacc                                                   <  2:517545/80‑1 (MQ=255)                    cccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGcc                                                      <  2:517546/77‑1 (MQ=255)                     ccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAg                                                 <  1:517549/81‑1 (MQ=255)                     ccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAg                                                 <  2:517549/81‑1 (MQ=255)                         aCTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCAt                                             >  2:517587/1‑81 (MQ=255)                            ctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCaa                                          >  1:517577/1‑81 (MQ=255)                              ttGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAata                                        >  1:517585/1‑81 (MQ=255)                                  cAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAAtattat                                    <  2:517550/81‑1 (MQ=255)                                   aaaCGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATattatt                                   <  2:517551/81‑1 (MQ=255)                                    aaCGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTg                                  >  2:517586/1‑81 (MQ=255)                                          ccgcAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGcc                            <  1:517553/81‑1 (MQ=255)                                             cAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGt                         <  2:517553/81‑1 (MQ=255)                                              aGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTg                        <  2:517554/81‑1 (MQ=255)                                                 cGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGc                       >  2:517589/1‑79 (MQ=255)                                                      gCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCt                >  2:517588/1‑81 (MQ=255)                                                        ggTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCttt              <  1:517556/81‑1 (MQ=255)                                                         gTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTtc             <  1:517557/81‑1 (MQ=255)                                                              cAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCt         <  2:517558/80‑1 (MQ=255)                                                              cAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCt         <  1:517558/80‑1 (MQ=255)                                                                    tGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCAt   >  2:517590/1‑80 (MQ=255)                                                                     gCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCAtt  <  2:517559/80‑1 (MQ=255)                                                                           |                                                                          CATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATT  >  NC_000913/3124664‑3124811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑