Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,388	T→C	I86I (ATT→ATC)	yghT →	putative ATP‑binding protein YghT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,388	0	T	C	100.0%	168.6 / NA	48	I86I (ATT→ATC)	yghT	putative ATP‑binding protein YghT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/14);  total (34/14)

CTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAAC  >  NC_000913/3134313‑3134465                                                                            |                                                                              cTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGc                                                                          >  1:519718/1‑81 (MQ=255)   tACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGt                                                                        >  2:519714/1‑81 (MQ=255)    aCATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCg                                                                         <  1:519684/79‑1 (MQ=255)        aaaaCAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTc                                                                   >  1:519731/1‑81 (MQ=255)         aaaCAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTc                                                                   <  2:519685/80‑1 (MQ=255)         aaaCAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTc                                                                   <  2:519684/80‑1 (MQ=255)         aaaCAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCg                                                                  >  2:519726/1‑81 (MQ=255)            cAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGccc                                                               <  1:519687/81‑1 (MQ=255)            cAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGccc                                                               >  1:519749/1‑81 (MQ=255)                     gAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTAc                                                      <  1:519688/81‑1 (MQ=255)                      aaaTCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACt                                                     <  2:519688/81‑1 (MQ=255)                      aaaTCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACt                                                     <  2:519689/81‑1 (MQ=255)                        aTCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTgc                                                   >  2:519729/1‑81 (MQ=255)                        aTCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTgc                                                   >  1:519721/1‑81 (MQ=255)                          cAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTgcgc                                                 >  1:519725/1‑81 (MQ=255)                          cAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTgcgc                                                 >  1:519738/1‑81 (MQ=255)                              tGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTc                                             >  1:519770/1‑81 (MQ=255)                              tGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTc                                             <  2:519690/81‑1 (MQ=255)                               gAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCg                                            >  1:519740/1‑81 (MQ=255)                                 aaaCAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGt                                           >  2:519719/1‑80 (MQ=255)                                 aaaCAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTc                                          <  1:519692/81‑1 (MQ=255)                                     aGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCatat                                      >  1:519735/1‑81 (MQ=255)                                      gCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATg                                     >  1:519745/1‑81 (MQ=255)                                        aaaaaaaTTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCt                                   <  2:519692/81‑1 (MQ=255)                                             aaTTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCt                              >  2:519747/1‑81 (MQ=255)                                              aTTGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTg                             >  1:519726/1‑81 (MQ=255)                                               ttGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTgt                            >  1:519723/1‑81 (MQ=255)                                                tGCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTgtc                           >  2:519735/1‑81 (MQ=255)                                                 gCCTGGACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTgtc                           <  2:519693/80‑1 (MQ=255)                                                      gACCAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTgtcgtcgtc                     >  2:519741/1‑81 (MQ=255)                                                         cAGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTcgcg                  >  1:519755/1‑81 (MQ=255)                                                          aGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCCTTCGTCATATGCTTGCCTGTCGTCGTCGCgg                 >  2:519743/1‑81 (MQ=255)                                                          aGTTCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCCTTCGTCATATGCTTGCCTGTCGTCGTCGCgg                 >  2:519721/1‑81 (MQ=255)                                                             tCCGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGt               >  1:519739/1‑80 (MQ=255)                                                              ccGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGtt              >  2:519745/1‑80 (MQ=255)                                                               cGGCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTaa            >  1:519751/1‑81 (MQ=255)                                                                ggCGCTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAAt           >  2:519736/1‑81 (MQ=255)                                                                  cgcTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTg         >  2:519728/1‑81 (MQ=255)                                                                  cgcTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTg         >  1:519732/1‑81 (MQ=255)                                                                   gcTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGt        >  2:519733/1‑81 (MQ=255)                                                                    cTGGTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGtt       >  2:519727/1‑81 (MQ=255)                                                                      ggTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTc      >  2:519751/1‑80 (MQ=255)                                                                       gTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCt     <  1:519697/80‑1 (MQ=255)                                                                       gTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCt     <  2:519695/80‑1 (MQ=255)                                                                       gTAATCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCt     <  1:519695/80‑1 (MQ=255)                                                                         aaTCACAGCGTTTGTCGTCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAac  >  1:519760/1‑81 (MQ=255)                                                                         aaTCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAac  >  2:519740/1‑81 (MQ=255)                                                                         aaTCACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAac  >  2:519757/1‑81 (MQ=255)                                                                            |                                                                              CTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAAC  >  NC_000913/3134313‑3134465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑