Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,139,574	A→T	E283V (GAA→GTA)	yghU →	disulfide reductase/organic hydroperoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,139,574	0	A	T	100.0%	127.8 / NA	36	E283V (GAA→GTA)	yghU	disulfide reductase/organic hydroperoxide reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/20);  total (16/20)

TTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGC  >  NC_000913/3139499‑3139647                                                                            |                                                                          ttcaCCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATa                                                                      <  2:520797/78‑1 (MQ=255) ttAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGAt                                                                       <  2:520796/80‑1 (MQ=255)    aCCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAg                                                                    <  1:520799/80‑1 (MQ=255)     ccGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCg                                                                  >  1:520845/1‑81 (MQ=255)      cGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGt                                                                 >  1:520832/1‑81 (MQ=255)       gCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTc                                                                <  1:520800/81‑1 (MQ=255)         aCCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAg                                                              >  2:520860/1‑81 (MQ=255)          ccAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAgg                                                             >  2:520846/1‑81 (MQ=255)                gACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAg                                                       <  1:520801/81‑1 (MQ=255)                 aCCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAgg                                                      <  1:520802/81‑1 (MQ=255)                    gCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATATGGTAGATAAGCGTCAGGGGTAAGGGtt                                                   >  2:520845/1‑81 (MQ=255)                    gCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGtt                                                   <  1:520803/81‑1 (MQ=255)                        aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgtg                                               >  2:520856/1‑81 (MQ=255)                        aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgt                                                <  1:520804/80‑1 (MQ=255)                             gCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtcgt                                          >  1:520841/1‑81 (MQ=255)                               aGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtcgtcg                                        <  2:520804/81‑1 (MQ=255)                                 ttGCATGAGCGCCATGACGCCAGTGATTTCGGGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCgca                                      <  2:520805/81‑1 (MQ=255)                                  tGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCgcag                                     >  1:520865/1‑81 (MQ=255)                                  tGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCgcag                                     >  2:520868/1‑81 (MQ=255)                                   gCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCgcat                                     >  1:520854/1‑79 (MQ=255)                                    cATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCgcagc                                    >  2:520841/1‑80 (MQ=255)                                     aTGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCaa                                  <  2:520806/81‑1 (MQ=255)                                        aGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGcc                               <  1:520808/81‑1 (MQ=255)                                        aGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGc                                >  1:520860/1‑80 (MQ=255)                                          cgcCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCAt                             <  2:520808/81‑1 (MQ=255)                                            ccATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATcc                           <  2:520809/81‑1 (MQ=255)                                             cATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCa                          <  2:520810/81‑1 (MQ=255)                                                 aCGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGcc                      <  2:520811/81‑1 (MQ=255)                                                    ccAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGa                   >  2:520858/1‑81 (MQ=255)                                                      aGTGATTTCGGGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATa                 <  1:520813/81‑1 (MQ=255)                                                       gTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATaa                <  1:520814/81‑1 (MQ=255)                                                           tttCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGTGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCg            <  2:520814/81‑1 (MQ=255)                                                               gagaCGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCa        >  2:520848/1‑81 (MQ=255)                                                               gagaCGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCa        >  2:520851/1‑81 (MQ=255)                                                                    gAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgccg   >  1:520863/1‑81 (MQ=255)                                                                     aaTACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgccgc  <  2:520815/81‑1 (MQ=255)                                                                            |                                                                          TTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGC  >  NC_000913/3139499‑3139647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑