Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,182,453	C→A	intergenic (‑19/‑97)	ygiD ← / → zupT	4,5‑DOPA dioxygenase extradiol/heavy metal divalent cation transporter ZupT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,453	0	C	A	100.0%	97.2 / NA	28	intergenic (‑19/‑97)	ygiD/zupT	4,5‑DOPA dioxygenase extradiol/heavy metal divalent cation transporter ZupT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/14);  total (14/14)

AAAACAATGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTG  >  NC_000913/3182377‑3182528                                                                             |                                                                            aaaaCAATGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCtt                                                                         >  2:530475/1‑81 (MQ=255) aaaaCAATGCTGGCATACGTGTTGAAGACATGATGATAACCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCt                                                                          <  2:530430/80‑1 (MQ=255)     cAATGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTc                                                                     <  2:530432/81‑1 (MQ=255)     cAATGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTc                                                                     <  2:530431/81‑1 (MQ=255)      aaTGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTcg                                                                    >  2:530455/1‑81 (MQ=255)       aTGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTcgg                                                                   >  1:530474/1‑81 (MQ=255)            ggCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAg                                                              >  1:530465/1‑81 (MQ=255)                  cGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGaa                                                        <  1:530434/81‑1 (MQ=255)                    tgtTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAAcc                                                      <  1:530435/81‑1 (MQ=255)                        gAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCgg                                                   <  1:530436/80‑1 (MQ=255)                          aGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGAt                                                 >  2:530466/1‑80 (MQ=255)                                 atgatATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAatga                                         >  2:530477/1‑81 (MQ=255)                                 atgatATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAatg                                          <  2:530436/80‑1 (MQ=255)                                  tgatATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAatgat                                        <  2:530438/81‑1 (MQ=255)                                  tgatATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAatgat                                        <  1:530438/81‑1 (MQ=255)                                   gatATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAatgat                                        >  2:530481/1‑80 (MQ=255)                                         cTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGatc                                 <  2:530440/81‑1 (MQ=255)                                         cTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGatc                                 <  1:530440/81‑1 (MQ=255)                                             aCTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGatcat                               >  2:530489/1‑79 (MQ=255)                                                   ggTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTAtt                       <  1:530442/81‑1 (MQ=255)                                                     tgtCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTAtttt                     <  2:530442/81‑1 (MQ=255)                                                      gtCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTAtttt                     >  1:530481/1‑80 (MQ=255)                                                             tGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATc              <  1:530444/80‑1 (MQ=255)                                                                   ccTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTg        >  2:530470/1‑80 (MQ=255)                                                                      cacaATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAgg    >  1:530485/1‑81 (MQ=255)                                                                       acaATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGt   >  2:530492/1‑81 (MQ=255)                                                                         aaTAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTg  >  2:530483/1‑80 (MQ=255)                                                                         aaTAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTg  >  2:530486/1‑80 (MQ=255)                                                                             |                                                                            AAAACAATGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTG  >  NC_000913/3182377‑3182528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑