Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,351	T→C	N39S (AAC→AGC)	ribB ←	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,351	0	T	C	100.0%	100.7 / NA	29	N39S (AAC→AGC)	ribB	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/19);  total (10/19)

CGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACG  >  NC_000913/3184277‑3184424                                                                           |                                                                          cGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCAc                                                                     <  1:530798/81‑1 (MQ=255)  gCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACg                                                                    <  1:530799/81‑1 (MQ=255)  gCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACg                                                                    >  2:530837/1‑81 (MQ=255)  gCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACg                                                                    >  1:530845/1‑81 (MQ=255)       cGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCtt                                                               <  2:530799/81‑1 (MQ=255)        gTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCtt                                                               >  2:530847/1‑80 (MQ=255)        gTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtc                                                              <  1:530801/81‑1 (MQ=255)         tGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtca                                                             >  2:530846/1‑81 (MQ=255)           gCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcat                                                            <  2:530801/80‑1 (MQ=255)             gAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcatcat                                                         >  1:530844/1‑81 (MQ=255)                     aGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCAc                                                  >  1:530853/1‑80 (MQ=255)                        gcCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCAtt                                              <  2:530804/81‑1 (MQ=255)                        gcCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCAtt                                              <  2:530803/81‑1 (MQ=255)                        gcCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCAtt                                              <  2:530802/81‑1 (MQ=255)                            tCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTacac                                          >  1:530842/1‑81 (MQ=255)                               gCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACcgc                                       >  1:530855/1‑81 (MQ=255)                                cTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACcg                                        <  1:530806/79‑1 (MQ=255)                                   aaCAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGt                                     <  1:530807/79‑1 (MQ=255)                                          aTGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgc                            <  2:530807/81‑1 (MQ=255)                                             gTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagc                         <  1:530809/81‑1 (MQ=255)                                                tCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagcgc                       <  2:530809/80‑1 (MQ=255)                                                    ccGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagcgcag                     <  1:530811/78‑1 (MQ=255)                                                    ccGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACgcagcgcagc                    <  1:530812/79‑1 (MQ=255)                                                      gggAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGt                <  1:530813/81‑1 (MQ=255)                                                        gAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGc              <  1:530814/81‑1 (MQ=255)                                                          aGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCAt            >  1:530864/1‑81 (MQ=255)                                                           gATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCAtt           <  1:530815/81‑1 (MQ=255)                                                               atatCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTc        >  1:530850/1‑80 (MQ=255)                                                                    aCCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACg  <  2:530815/81‑1 (MQ=255)                                                                           |                                                                          CGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACG  >  NC_000913/3184277‑3184424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑