Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,190,185	T→C	L102P (CTT→CCT)	yqiH →	putative fimbrial chaperone YqiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,190,185	0	T	C	100.0%	82.3 / NA	24	L102P (CTT→CCT)	yqiH	putative fimbrial chaperone YqiH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/10);  total (14/10)

CTGTTCCTCCAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATG  >  NC_000913/3190107‑3190261                                                                               |                                                                             cTGTTCCTCCAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTc                                                                            <  2:531856/81‑1 (MQ=255)      cctccAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTaa                                                                        <  1:531858/80‑1 (MQ=255)           aaTGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACag                                                                  <  1:531859/81‑1 (MQ=255)              ggTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACagag                                                                >  2:531909/1‑80 (MQ=255)                tGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGaaa                                                             >  1:531886/1‑81 (MQ=255)                  cggttAAAACCCAGTGGGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACg                                                           <  1:531860/78‑1 (MQ=255)                  cGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAAc                                                            <  1:531861/80‑1 (MQ=255)                   gTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGc                                                          >  2:531891/1‑81 (MQ=255)                   gTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGc                                                          >  1:531888/1‑81 (MQ=255)                    tttAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGct                                                         >  1:531887/1‑81 (MQ=255)                    tttAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGct                                                         <  1:531863/81‑1 (MQ=255)                    tttAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGct                                                         <  1:531862/81‑1 (MQ=255)                                  aGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACg                                           >  1:531889/1‑81 (MQ=255)                                     aaaTTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGc                                        >  2:531901/1‑81 (MQ=255)                                      aaTTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCg                                       <  2:531863/81‑1 (MQ=255)                                       aTTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGc                                        >  1:531896/1‑79 (MQ=255)                                                  aaTGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGcc                           >  2:531890/1‑81 (MQ=255)                                                   aTGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGcc                           >  2:531907/1‑80 (MQ=255)                                                     gATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCa                         >  2:531959/1‑80 (MQ=255)                                                           tCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCaaaa                      >  1:531893/1‑77 (MQ=255)                                                                         cTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtgtg     <  1:531865/80‑1 (MQ=255)                                                                         cTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGa    >  1:531895/1‑81 (MQ=255)                                                                          tCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGAt   >  1:531899/1‑81 (MQ=255)                                                                            aaCCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATg  <  1:531866/80‑1 (MQ=255)                                                                               |                                                                             CTGTTCCTCCAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATG  >  NC_000913/3190107‑3190261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑