Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,200,368	G→A	Q200* (CAG→TAG)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,200,368	0	G	A	100.0%	150.9 / NA	42	Q200* (CAG→TAG)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/25);  total (17/25)

AGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCG  >  NC_000913/3200292‑3200442                                                                             |                                                                           aGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTacgc                                                                         >  2:534064/1‑80 (MQ=255) aGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTacgc                                                                         >  1:534051/1‑80 (MQ=255)  gCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCa                                                                       <  2:534003/81‑1 (MQ=255)  gCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCa                                                                       <  1:534003/81‑1 (MQ=255)   ccccTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAg                                                                      <  2:534004/81‑1 (MQ=255)     ccTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAgat                                                                    <  2:534005/81‑1 (MQ=255)         ttCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAgatga                                                                  >  2:534059/1‑79 (MQ=255)            cacaTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGcc                                                             <  2:534006/81‑1 (MQ=255)             acaTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCa                                                            <  1:534008/81‑1 (MQ=255)              caTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCAc                                                           <  2:534008/81‑1 (MQ=255)                tCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCAcgc                                                         <  1:534010/81‑1 (MQ=255)                  ggCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCAcgcg                                                        <  1:534011/80‑1 (MQ=255)                  ggCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACgcgc                                                       >  1:534082/1‑81 (MQ=255)                       ttGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGcgccgc                                                    <  1:534012/79‑1 (MQ=255)                        tGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCtt                                                  <  1:534013/80‑1 (MQ=255)                             cAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCt                                              >  1:534071/1‑79 (MQ=255)                              aaCATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAg                                           <  1:534014/81‑1 (MQ=255)                                 aTGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCTGATGATAGCCACGCGCCGCTTTGCTCAGGCt                                        >  2:534071/1‑81 (MQ=255)                                 aTGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCt                                        >  2:534082/1‑81 (MQ=255)                                 aTGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCt                                        <  1:534015/81‑1 (MQ=255)                                    cAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGcc                                     <  1:534016/81‑1 (MQ=255)                                      aaaTGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCt                                   <  1:534018/81‑1 (MQ=255)                                      aaaTGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCt                                   <  1:534019/81‑1 (MQ=255)                                      aaaTGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCt                                   <  1:534017/81‑1 (MQ=255)                                       aaTGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTg                                  <  1:534020/81‑1 (MQ=255)                                          ggtggtCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCg                               >  1:534088/1‑81 (MQ=255)                                            tggtCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGt                              >  1:534069/1‑80 (MQ=255)                                              gtaggTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAAt                           <  1:534021/78‑1 (MQ=255)                                              gtCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAAt                           <  2:534021/81‑1 (MQ=255)                                              gtCGGTTCGATCTCACGCTCTGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTaac                           >  2:534116/1‑80 (MQ=255)                                                 ggTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATcc                         <  2:534022/80‑1 (MQ=255)                                                   tttGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGt                      >  1:534095/1‑81 (MQ=255)                                                   tttGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGt                      >  1:534091/1‑81 (MQ=255)                                                    ttGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGtt                     >  1:534081/1‑81 (MQ=255)                                                       aTTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGc                  >  1:534077/1‑81 (MQ=255)                                                       aTTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGc                  <  1:534024/81‑1 (MQ=255)                                                         ttCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGa                >  1:534080/1‑81 (MQ=255)                                                          tCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGa                >  2:534073/1‑80 (MQ=255)                                                            aCGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCCCAGGCTGCCCTGGCGTAATCCGGTTTGCGATAc             >  2:534088/1‑81 (MQ=255)                                                                      ttGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTc   <  2:534025/81‑1 (MQ=255)                                                                      ttGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTc   >  1:534073/1‑81 (MQ=255)                                                                      ttGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTc   <  1:534027/81‑1 (MQ=255)                                                                      ttGCCCTACGACAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTc   <  2:534024/81‑1 (MQ=255)                                                                       tGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCg  >  2:534095/1‑81 (MQ=255)                                                                             |                                                                           AGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCG  >  NC_000913/3200292‑3200442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑