Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,553	C→T	P61P (CCC→CCT)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,553	0	C	T	100.0%	141.8 / NA	39	P61P (CCC→CCT)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/24);  total (15/24)

GTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAA  >  NC_000913/3207477‑3207622                                                                             |                                                                      gTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATc                                                                    <  2:535718/80‑1 (MQ=255)      cgccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTgcg                                                               >  2:535779/1‑80 (MQ=255)       gccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCa                                                             <  1:535722/81‑1 (MQ=255)       gccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCa                                                             <  2:535719/81‑1 (MQ=255)       gccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCa                                                             <  1:535721/81‑1 (MQ=255)         cgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCaaa                                                           <  1:535723/81‑1 (MQ=255)         cgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCaa                                                            >  2:535775/1‑80 (MQ=255)              gATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACgg                                                      >  1:535769/1‑81 (MQ=255)              gATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACg                                                       <  2:535723/80‑1 (MQ=255)               aTCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGc                                                     >  1:535784/1‑81 (MQ=255)                     cTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACaaa                                               >  1:535783/1‑81 (MQ=255)                        aaaCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATg                                             <  2:535724/80‑1 (MQ=255)                               cGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATgg                                     <  1:535726/81‑1 (MQ=255)                               cGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATg                                      <  1:535727/80‑1 (MQ=255)                                  tCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtct                                  >  2:535790/1‑81 (MQ=255)                                  tCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtct                                  >  2:535773/1‑81 (MQ=255)                                     cTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCg                               <  1:535728/81‑1 (MQ=255)                                        aCGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTc                             <  2:535729/80‑1 (MQ=255)                                        aCGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTc                             <  2:535728/80‑1 (MQ=255)                                           aTCTCAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGcc                         >  1:535775/1‑81 (MQ=255)                                             ctcAACGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGccc                        >  2:535777/1‑80 (MQ=255)                                                aaCGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACa                    <  1:535731/81‑1 (MQ=255)                                                  cGGCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAc                  <  1:535732/81‑1 (MQ=255)                                                   ggCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAcc                 <  1:535733/81‑1 (MQ=255)                                                   ggCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAc                  >  1:535792/1‑80 (MQ=255)                                                   ggCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAc                  >  2:535784/1‑80 (MQ=255)                                                    gCAAAGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCa                <  1:535734/81‑1 (MQ=255)                                                      aaaGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAg               >  2:535781/1‑80 (MQ=255)                                                        aGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAt            >  1:535777/1‑81 (MQ=255)                                                        aGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAt            <  2:535734/81‑1 (MQ=255)                                                        aGCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAt            <  1:535736/81‑1 (MQ=255)                                                         gCGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATg           <  1:535737/81‑1 (MQ=255)                                                          cGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGc          >  2:535794/1‑81 (MQ=255)                                                          cGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGc          >  2:535792/1‑81 (MQ=255)                                                          cGATTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGc          <  1:535738/81‑1 (MQ=255)                                                            aTTTTCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGt        <  1:535739/81‑1 (MQ=255)                                                               ttCCACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTAt      <  1:535740/80‑1 (MQ=255)                                                                 ccACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATgg    <  2:535740/80‑1 (MQ=255)                                                                  cACGCTGGCCCTATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGaa  <  1:535742/81‑1 (MQ=255)                                                                             |                                                                      GTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAA  >  NC_000913/3207477‑3207622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑