| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,243,465 | C→A | R426L (CGC→CTC) | uxaC ← | D‑glucoronate/D‑galacturonate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,243,465 | 0 | C | A | 97.0% | 115.0 / NA | 33 | R426L (CGC→CTC) | uxaC | D‑glucoronate/D‑galacturonate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (15/17); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACC > NC_000913/3243390‑3243541 | tcGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAg > 1:544088/1‑81 (MQ=255) cGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAg > 2:544088/1‑80 (MQ=255) cGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAg > 1:544078/1‑80 (MQ=255) gCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTa > 2:544093/1‑81 (MQ=255) gCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTa < 2:544020/81‑1 (MQ=255) cccAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTAt > 1:544083/1‑81 (MQ=255) ccAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTAtt < 2:544021/81‑1 (MQ=255) gcagGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGt > 2:544081/1‑81 (MQ=255) agGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGa < 2:544022/81‑1 (MQ=255) gTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACg < 2:544023/81‑1 (MQ=255) tCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTa < 2:544024/80‑1 (MQ=255) cgccgGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGa < 2:544025/81‑1 (MQ=255) gccgGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTTTATGAc < 2:544027/81‑1 (MQ=255) gccgGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGAc < 1:544027/81‑1 (MQ=255) gccgGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAACGCGGAGGAAGTATTCGTGACGGGTGTATGa > 2:544075/1‑80 (MQ=255) gTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGa < 2:544028/81‑1 (MQ=255) ttCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGc > 2:544076/1‑80 (MQ=255) gCCCGCCTCCACCCAGCGACGGATCATCTGGCTCAGAATGCGGCGGAAGTCTTCGTTACGGGTGTATGACAGGAAGCTACg > 2:544097/1‑81 (MQ=255) tCCACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgt > 1:544093/1‑79 (MQ=255) ccACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGGCAGGAAGCTACGGCTgtc < 2:544030/79‑1 (MQ=255) ccACCCAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtc < 2:544029/79‑1 (MQ=255) cccAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtcagt > 1:544108/1‑79 (MQ=255) ccAGCGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGtcagtcag < 2:544031/81‑1 (MQ=255) cGACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATa < 1:544033/81‑1 (MQ=255) gACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATAc > 1:544111/1‑81 (MQ=255) gACCGATCATCTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATAc > 1:544102/1‑81 (MQ=255) atcTGGCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAg > 1:544084/1‑81 (MQ=255) gCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGc < 2:544033/77‑1 (MQ=255) gCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGc < 1:544035/80‑1 (MQ=255) gCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCt > 1:544087/1‑81 (MQ=255) gCACAGAATGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCt > 2:544084/1‑81 (MQ=255) aaTGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagcag < 1:544036/81‑1 (MQ=255) tGCGGAGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGAc < 1:544037/81‑1 (MQ=255) cggaggAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGAcc > 2:544113/1‑80 (MQ=255) | TCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACC > NC_000913/3243390‑3243541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |