Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,257,401	G→T	H204N (CAT→AAT)	cyuP ←	putative D/L‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,257,401	0	G	T	100.0%	150.6 / NA	42	H204N (CAT→AAT)	cyuP	putative D/L‑serine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/22);  total (20/22)

TAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTGA  >  NC_000913/3257329‑3257478                                                                         |                                                                              tAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTc                                                                       >  1:547174/1‑81 (MQ=255)   aaCGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTccc                                                                     <  2:547109/81‑1 (MQ=255)   aaCGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTcc                                                                      >  2:547169/1‑80 (MQ=255)   aaCGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTcc                                                                      >  1:547162/1‑80 (MQ=255)     cGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCccac                                                                   <  1:547112/81‑1 (MQ=255)     cGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCccac                                                                   <  2:547110/81‑1 (MQ=255)         aGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATc                                                               >  2:547158/1‑81 (MQ=255)         aGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATc                                                               >  2:547143/1‑81 (MQ=255)          gCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATc                                                               >  2:547154/1‑80 (MQ=255)          gCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCg                                                              <  2:547112/81‑1 (MQ=255)             taataaTGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGaca                                                           >  2:547162/1‑81 (MQ=255)             taataaTGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGaca                                                           >  1:547160/1‑81 (MQ=255)                     gCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAgc                                                   <  2:547113/81‑1 (MQ=255)                          tttCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGcgccg                                               <  2:547114/80‑1 (MQ=255)                             cACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGac                                           >  2:547164/1‑81 (MQ=255)                                    agcCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACcag                                     >  2:547176/1‑80 (MQ=255)                                    agcCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACcag                                     >  2:547178/1‑80 (MQ=255)                                         cAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCtt                               <  1:547116/81‑1 (MQ=255)                                           gcggcgGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTgg                             >  1:547185/1‑81 (MQ=255)                                             ggcggTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGt                            >  1:547164/1‑80 (MQ=255)                                             ggcggTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGt                            >  1:547161/1‑80 (MQ=255)                                               cggTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATTGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAg                         >  1:547178/1‑81 (MQ=255)                                                ggTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGc                        <  2:547116/81‑1 (MQ=255)                                                 gTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCa                       <  1:547118/81‑1 (MQ=255)                                                    gCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCa                    <  1:547119/81‑1 (MQ=255)                                                     cGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCAt                   <  1:547120/81‑1 (MQ=255)                                                     cGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCAt                   <  1:547121/81‑1 (MQ=255)                                                     cGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCAt                   <  1:547122/81‑1 (MQ=255)                                                      gAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATg                  >  2:547182/1‑81 (MQ=255)                                                      gAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATg                  >  1:547168/1‑81 (MQ=255)                                                        aCCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGcc                >  2:547180/1‑81 (MQ=255)                                                           gACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGt             <  1:547123/81‑1 (MQ=255)                                                            aCGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTc            >  2:547181/1‑81 (MQ=255)                                                               ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaa         <  1:547126/81‑1 (MQ=255)                                                               ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaa         <  1:547124/81‑1 (MQ=255)                                                               ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaa         <  2:547124/81‑1 (MQ=255)                                                               ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGa          >  2:547174/1‑80 (MQ=255)                                                               ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCACCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaa         <  1:547127/81‑1 (MQ=255)                                                                  taCAGATTCCACATACCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAtt      <  1:547128/81‑1 (MQ=255)                                                                    cAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAtttt    <  2:547128/81‑1 (MQ=255)                                                                    cAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAttt     <  2:547129/80‑1 (MQ=255)                                                                      gATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTGa  <  2:547130/81‑1 (MQ=255)                                                                         |                                                                              TAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTGA  >  NC_000913/3257329‑3257478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑