Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,265,761	C→A	V90F (GTT→TTT)	tdcB ←	catabolic threonine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,265,761	0	C	A	100.0%	114.6 / NA	32	V90F (GTT→TTT)	tdcB	catabolic threonine dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/16);  total (16/16)

CGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATTTAT  >  NC_000913/3265684‑3265838                                                                              |                                                                              cGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAaccc                                                                            <  2:549000/81‑1 (MQ=255)         tGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCAt                                                                    >  1:549042/1‑81 (MQ=255)          ggATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATg                                                                   >  2:549042/1‑81 (MQ=255)           gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATgg                                                                  >  1:549035/1‑81 (MQ=255)           gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATg                                                                   <  2:549001/80‑1 (MQ=255)            aTTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGt                                                                 <  2:549003/81‑1 (MQ=255)            aTTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGt                                                                 <  2:549002/81‑1 (MQ=255)             ttttGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGtt                                                                <  1:549005/81‑1 (MQ=255)             ttttGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGtt                                                                <  2:549005/81‑1 (MQ=255)                    gcACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCg                                                           >  2:549052/1‑79 (MQ=255)                     cACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCg                                                           >  2:549039/1‑78 (MQ=255)                        cTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGaa                                                      >  2:549036/1‑80 (MQ=255)                         ttttGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGaa                                                      >  1:549039/1‑79 (MQ=255)                                     cacTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGcc                                        <  2:549006/81‑1 (MQ=255)                                       cTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCtt                                      <  1:549008/81‑1 (MQ=255)                                        tttACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCttt                                     >  1:549046/1‑81 (MQ=255)                                          tACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGc                                   >  1:549061/1‑81 (MQ=255)                                              gTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGttt                               >  2:549061/1‑81 (MQ=255)                                                 gATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTc                            >  2:549049/1‑81 (MQ=255)                                                  aTACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTcc                           <  2:549008/81‑1 (MQ=255)                                                  aTACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTcc                           <  2:549009/81‑1 (MQ=255)                                                   tACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCg                          <  2:549010/81‑1 (MQ=255)                                                        aGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCg                     <  2:549011/81‑1 (MQ=255)                                                            tCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGt                   >  1:549055/1‑79 (MQ=255)                                                             cgcgCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg                >  1:549057/1‑81 (MQ=255)                                                               cgcAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTg              <  1:549014/81‑1 (MQ=255)                                                               cgcAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTg              <  1:549013/81‑1 (MQ=255)                                                                 cAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGaa            >  1:549047/1‑81 (MQ=255)                                                                  aGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAAc           >  1:549059/1‑81 (MQ=255)                                                                    gagaGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACtt         <  1:549015/81‑1 (MQ=255)                                                                      gagGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTaa       <  1:549016/81‑1 (MQ=255)                                                                       agGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAAt      >  1:549056/1‑81 (MQ=255)                                                                        gggAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAAtt     <  1:549017/81‑1 (MQ=255)                                                                           aaaaCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATttat  <  2:549017/81‑1 (MQ=255)                                                                              |                                                                              CGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATTTAT  >  NC_000913/3265684‑3265838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑