| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,269,345 | A→C | I311L (ATC→CTC) | yhaC → | uncharacterized protein YhaC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,269,345 | 0 | A | C | 84.2% | 52.4 / 3.0 | 19 | I311L (ATC→CTC) | yhaC | uncharacterized protein YhaC |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base C (9/7); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATA > NC_000913/3269271‑3269425 | aTTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATACTCAtt > 2:549569/1‑80 (MQ=255) agaTGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATg < 2:549539/81‑1 (MQ=255) aTGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCt < 1:549541/81‑1 (MQ=255) aaGAATTCTCATCAGAAAAAATATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATc > 1:549560/1‑81 (MQ=255) aaTTCTCATCAGAAAAAATATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTgg > 1:549569/1‑81 (MQ=255) catcaGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTcc > 2:549552/1‑81 (MQ=255) atcaGAAAAAATATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGgtcgtc < 2:549541/79‑1 (MQ=255) aaaaaaTATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCaaa < 1:549543/81‑1 (MQ=255) atGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATtata > 1:549574/1‑81 (MQ=255) atGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATtata > 2:549571/1‑81 (MQ=255) aTTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTa > 2:549564/1‑81 (MQ=255) aCATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATg > 1:549568/1‑80 (MQ=255) aCATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATg < 1:549544/80‑1 (MQ=255) cATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATgg < 1:549545/80‑1 (MQ=255) aTCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAt > 2:549565/1‑81 (MQ=255) gATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAttt < 2:549545/80‑1 (MQ=255) gATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAttt > 2:549570/1‑80 (MQ=255) gATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTa < 2:549546/81‑1 (MQ=255) tataATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTaaa > 2:549572/1‑81 (MQ=255) aTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATa < 1:549548/81‑1 (MQ=255) | ATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATA > NC_000913/3269271‑3269425 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |