Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	319,137	C→G	A289P (GCG→CCG)	rclA ←	putative pyridine nucleotide‑disulfide oxidoreductase RclA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	319,137	0	C	G	100.0%	82.1 / NA	24	A289P (GCG→CCG)	rclA	putative pyridine nucleotide‑disulfide oxidoreductase RclA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (16/8);  total (16/8)

GTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCA  >  NC_000913/319059‑319213                                                                               |                                                                             gTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGgcc                                                                            <  1:66344/81‑1 (MQ=255)        atcaCGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATAtt                                                                     >  2:66376/1‑81 (MQ=255)                 aaTGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCggt                                                            <  1:66345/81‑1 (MQ=255)                  aTGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCggtg                                                           >  2:66395/1‑81 (MQ=255)                      ggTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTAt                                                       >  1:66399/1‑81 (MQ=255)                           tcatcCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAAt                                                  >  2:66408/1‑81 (MQ=255)                            catcCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATc                                                 >  1:66370/1‑81 (MQ=255)                               ccAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCt                                              >  2:66388/1‑81 (MQ=255)                                cAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCtt                                             <  1:66347/81‑1 (MQ=255)                                cAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCtt                                             >  2:66390/1‑81 (MQ=255)                                cAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCt                                              <  1:66346/80‑1 (MQ=255)                                     atatGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCaa                                        >  2:66384/1‑81 (MQ=255)                                     atatGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCaa                                        >  2:66386/1‑81 (MQ=255)                                                aaTTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGcc                             <  1:66349/81‑1 (MQ=255)                                                aaTTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGc                              <  1:66348/80‑1 (MQ=255)                                                  ttGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGcccc                           <  1:66350/81‑1 (MQ=255)                                                    gCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCcg                          <  1:66351/80‑1 (MQ=255)                                                     cAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCcgcg                        >  1:66391/1‑81 (MQ=255)                                                      aGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCgcgc                       >  2:66409/1‑81 (MQ=255)                                                          cgccgGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGt                   >  1:66396/1‑81 (MQ=255)                                                             cgGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTa                >  1:66410/1‑81 (MQ=255)                                                                    aTCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGa          >  2:66381/1‑80 (MQ=255)                                                                    aTCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGa          >  2:66397/1‑80 (MQ=255)                                                                         ccATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCgg    <  1:66352/81‑1 (MQ=255)                                                                           aTCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCa  >  2:66400/1‑81 (MQ=255)                                                                               |                                                                             GTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCA  >  NC_000913/319059‑319213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑