Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,310,694	C→T	D159N (GAC→AAC)	pnp ←	polynucleotide phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,310,694	0	C	T	100.0%	116.1 / NA	32	D159N (GAC→AAC)	pnp	polynucleotide phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

CTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGC  >  NC_000913/3310619‑3310769                                                                            |                                                                            cTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGa                                                                        <  2:558950/81‑1 (MQ=255)     aGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGt                                                                     >  2:559001/1‑80 (MQ=255)         ccGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAAcc                                                                 <  1:558952/80‑1 (MQ=255)              aacaacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTAcgcg                                                           <  2:558952/81‑1 (MQ=255)                 aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGgc                                                        <  1:558954/81‑1 (MQ=255)                    cAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGgcagc                                                     <  2:558954/81‑1 (MQ=255)                       aTCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCAc                                                   >  1:558999/1‑80 (MQ=255)                        tCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCAcc                                                  >  1:559016/1‑80 (MQ=255)                           aGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATc                                              <  1:558956/81‑1 (MQ=255)                               tGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGc                                          >  1:559018/1‑81 (MQ=255)                                      tttCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCAtt                                      <  2:558956/78‑1 (MQ=255)                                        tCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGa                                    <  1:558958/78‑1 (MQ=255)                                         cAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACg                                 >  1:559003/1‑80 (MQ=255)                                              cGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAc                           >  2:559021/1‑81 (MQ=255)                                              cGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAc                           >  2:559016/1‑81 (MQ=255)                                               gTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAcc                          <  2:558958/81‑1 (MQ=255)                                               gTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAc                           >  1:559010/1‑80 (MQ=255)                                                tCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAc                           >  1:559026/1‑79 (MQ=255)                                                    gAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACcaga                       >  2:559008/1‑79 (MQ=255)                                                     aGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACcagacag                    <  2:558959/81‑1 (MQ=255)                                                      gTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACcagaca                     >  1:559014/1‑79 (MQ=255)                                                       tCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCagacagag                  <  2:558960/81‑1 (MQ=255)                                                       tCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCagacagag                  <  2:558961/81‑1 (MQ=255)                                                          ggTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCagacagagaca               <  1:558963/81‑1 (MQ=255)                                                           gTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCagacagagacag              >  1:559013/1‑81 (MQ=255)                                                            ttCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAgc             >  1:559011/1‑81 (MQ=255)                                                               aGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCt          >  2:559024/1‑81 (MQ=255)                                                                gtacgtacTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTg         >  2:559020/1‑81 (MQ=255)                                                                 tacgtacTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGc        <  1:558964/81‑1 (MQ=255)                                                                 tacgtacTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGc        >  1:559012/1‑81 (MQ=255)                                                                       cTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGc  <  2:558965/81‑1 (MQ=255)                                                                       cTGGTTATTGATGTAACCTACGCGGGCAGAACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAg   <  2:558964/80‑1 (MQ=255)                                                                            |                                                                            CTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGC  >  NC_000913/3310619‑3310769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑