Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,313,786	C→T	L743L (CTG→CTA)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,313,786	0	C	T	100.0%	179.0 / NA	49	L743L (CTG→CTA)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/27);  total (22/27)

GGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTC  >  NC_000913/3313708‑3313861                                                                               |                                                                            ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg                                                                           >  2:559779/1‑81 (MQ=255) ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg                                                                           >  2:559747/1‑81 (MQ=255) ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg                                                                           >  2:559746/1‑81 (MQ=255) ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg                                                                           >  1:559763/1‑81 (MQ=255)  gAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg                                                                           >  2:559782/1‑80 (MQ=255)       gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGc                                                                     <  2:559697/81‑1 (MQ=255)        taACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCg                                                                    >  1:559764/1‑81 (MQ=255)         aaCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGt                                                                   >  1:559766/1‑81 (MQ=255)             cAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGa                                                               <  2:559698/81‑1 (MQ=255)              aGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAc                                                              >  1:559775/1‑81 (MQ=255)                aTCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAcgc                                                            <  1:559700/81‑1 (MQ=255)                 tcaaGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAcgcc                                                           <  2:559700/78‑1 (MQ=255)                   cAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAcgccg                                                          >  1:559770/1‑80 (MQ=255)                          tCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCa                                                    <  2:559701/79‑1 (MQ=255)                           ccGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAggg                                                 >  1:559774/1‑81 (MQ=255)                            cGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGt                                                <  1:559703/81‑1 (MQ=255)                             gCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTg                                               >  1:559760/1‑81 (MQ=255)                              cTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTgg                                              >  1:559771/1‑81 (MQ=255)                                 cAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCg                                            <  1:559704/80‑1 (MQ=255)                                 cAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGt                                           >  2:559767/1‑81 (MQ=255)                                  aaTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTc                                          <  1:559705/81‑1 (MQ=255)                                   aTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCg                                         <  2:559705/81‑1 (MQ=255)                                    tCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCgg                                        >  1:559776/1‑81 (MQ=255)                                      aCTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGtt                                      >  1:559767/1‑81 (MQ=255)                                      aCTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGt                                       <  2:559706/80‑1 (MQ=255)                                       cTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGttt                                     <  1:559708/81‑1 (MQ=255)                                         ttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCg                                   >  2:559771/1‑81 (MQ=255)                                          tACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTc                                    <  2:559708/79‑1 (MQ=255)                                          tACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCgg                                  >  1:559759/1‑81 (MQ=255)                                          tACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCg                                   <  1:559710/80‑1 (MQ=255)                                          tACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCg                                   <  2:559710/80‑1 (MQ=255)                                              tGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGAt                              <  1:559712/81‑1 (MQ=255)                                                 agagGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacc                           <  1:559713/81‑1 (MQ=255)                                                   agGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccac                         >  2:559768/1‑81 (MQ=255)                                                     gCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCt                       <  2:559713/81‑1 (MQ=255)                                                     gCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCt                       <  2:559714/81‑1 (MQ=255)                                                       aTCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTAc                     <  2:559715/81‑1 (MQ=255)                                                       aTCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTAc                     <  2:559716/81‑1 (MQ=255)                                                             acgtacgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGa               <  1:559718/81‑1 (MQ=255)                                                              cgtacgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGaa              >  2:559776/1‑81 (MQ=255)                                                                tacgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc            >  1:559781/1‑81 (MQ=255)                                                                tacgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc            <  1:559721/81‑1 (MQ=255)                                                                tacgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc            <  2:559719/81‑1 (MQ=255)                                                                tacgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc            <  1:559719/81‑1 (MQ=255)                                                                 acgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc            >  2:559772/1‑80 (MQ=255)                                                                  cgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCga          >  1:559792/1‑81 (MQ=255)                                                                   gtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat         <  2:559721/81‑1 (MQ=255)                                                                        aGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCt    <  1:559723/81‑1 (MQ=255)                                                                          ccAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTc  <  1:559724/81‑1 (MQ=255)                                                                               |                                                                            GGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTC  >  NC_000913/3313708‑3313861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑