| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,314,620 | T→C | V465V (GTA→GTG) | infB ← | translation initiation factor IF‑2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,314,620 | 0 | T | C | 97.6% | 144.1 / NA | 42 | V465V (GTA→GTG) | infB | translation initiation factor IF‑2 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (25/16); minor base A (0/1); total (25/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTG > NC_000913/3314542‑3314695 | tACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCAc > 2:559988/1‑81 (MQ=255) cgccgcTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATg < 2:559945/79‑1 (MQ=255) gccgcTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTcc < 1:559947/81‑1 (MQ=255) gccgcTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTcc > 2:560017/1‑81 (MQ=255) gccgcTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTcc < 2:559947/81‑1 (MQ=255) cgcTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGt > 2:559981/1‑81 (MQ=255) cTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTg < 2:559948/81‑1 (MQ=255) cgcgTGCTGGATTGCTTCGATGGTCTGCGGGATCACACCGTCGTAGGCAGCAACAACCAGGACCACGATGTCCGTTGCCt < 2:559949/80‑1 (MQ=255) cgcgTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCt < 1:559951/80‑1 (MQ=255) gcgTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTgc > 2:559996/1‑81 (MQ=255) gcgTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTgc > 1:560021/1‑81 (MQ=255) gTGCTGGATTGCTTCGATGGTCTGCGGCATCACTCCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTgcg > 2:560015/1‑80 (MQ=255) cTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGcac > 2:559994/1‑80 (MQ=255) tGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGcacca > 1:560009/1‑81 (MQ=255) gATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACg < 2:559951/81‑1 (MQ=255) tGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAg > 1:559990/1‑80 (MQ=255) gATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCAt > 1:560020/1‑81 (MQ=255) gATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCAt < 2:559952/81‑1 (MQ=255) tGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCAtt > 1:559994/1‑80 (MQ=255) ggTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTg > 1:559992/1‑80 (MQ=255) gCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTa > 2:560005/1‑80 (MQ=255) cGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGt < 1:559956/78‑1 (MQ=255) cGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTaa < 1:559954/80‑1 (MQ=255) cGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTaa < 1:559955/80‑1 (MQ=255) ggCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAAc < 2:559956/81‑1 (MQ=255) cATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAAcgc > 2:560002/1‑81 (MQ=255) acacCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACgcggcg > 1:560008/1‑81 (MQ=255) ccGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGc > 2:560010/1‑81 (MQ=255) cgtcgGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCg > 2:560016/1‑81 (MQ=255) cgtcgGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCg > 1:560031/1‑81 (MQ=255) cgGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCgggg > 1:560010/1‑81 (MQ=255) cgGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCgggg > 2:560025/1‑81 (MQ=255) ggCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGgt > 1:560000/1‑81 (MQ=255) gcagaaACAACAAGGACAACGATGTCCGTTGGCTGAGAAGAACGAGAACGCATTGAAGTAAACGCGGCGTGCCCCGGGgtg < 1:897230/81‑1 (MQ=255) agcaACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTc > 1:560005/1‑81 (MQ=255) gcaACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTc > 2:560024/1‑80 (MQ=255) aacaacCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAg > 2:560027/1‑81 (MQ=255) aacCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAgg > 1:560013/1‑79 (MQ=255) aacCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGaa < 2:559958/81‑1 (MQ=255) aacCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGaa < 1:559958/81‑1 (MQ=255) cAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGt < 1:559960/81‑1 (MQ=255) aGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTg < 2:559960/81‑1 (MQ=255) | TACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTG > NC_000913/3314542‑3314695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |