Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,324,584	C→A	G110C (GGC→TGC)	folP ←	dihydropteroate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,324,584	0	C	A	100.0%	117.9 / NA	33	G110C (GGC→TGC)	folP	dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/16);  total (17/16)

CCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGCAATTG  >  NC_000913/3324506‑3324661                                                                               |                                                                              ccggtaaaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCaaa                                                                             <  1:562237/81‑1 (MQ=255)   ggtaaaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACt                                                                           >  1:562280/1‑81 (MQ=255)    gtgaaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACtt                                                                          <  2:562237/78‑1 (MQ=255)       aaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTc                                                                        <  1:562239/80‑1 (MQ=255)       aaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCg                                                                       <  1:562240/81‑1 (MQ=255)         ccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCt                                                                     <  1:562241/81‑1 (MQ=255)          cggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCt                                                                     >  1:562242/1‑80 (MQ=255)            gtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATTATTAATAATGTGAGCGCAAACTTTCGCTGAc                                                                  >  2:562289/1‑81 (MQ=255)                cTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCAc                                                              >  2:562288/1‑81 (MQ=255)                       cgccTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGAc                                                       <  2:562242/81‑1 (MQ=255)                        gccTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACt                                                      <  2:562243/81‑1 (MQ=255)                         ccTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACtt                                                     >  1:562284/1‑81 (MQ=255)                              agagCGCCCGGTTCGGATAGGGAGCGGATATCATCAACAATGTGAGCGAAAACTTTCGCTGACTCATGGATGACTACTGGt                                                >  2:562295/1‑81 (MQ=255)                                        gTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTAt                                      >  1:562306/1‑81 (MQ=255)                                         ttCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATc                                     >  1:562323/1‑81 (MQ=255)                                         ttCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATc                                     <  2:562244/81‑1 (MQ=255)                                           cGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCg                                    <  2:562245/80‑1 (MQ=255)                                            ggAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGa                                   <  2:562246/80‑1 (MQ=255)                                             gAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACt                                 >  1:562298/1‑81 (MQ=255)                                               aaGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACt                                 >  2:562308/1‑79 (MQ=255)                                               aaGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACt                                 >  2:562287/1‑79 (MQ=255)                                               aaGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTga                               <  1:562248/81‑1 (MQ=255)                                                    aGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATc                           >  2:562296/1‑80 (MQ=255)                                                       ggATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGa                       >  1:562304/1‑81 (MQ=255)                                                       ggATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGa                       >  1:562295/1‑81 (MQ=255)                                                           atCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTc                   >  1:562294/1‑81 (MQ=255)                                                            tCATTAATAATGTGAGCGAAAACTTTCGCTGACTAACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCg                  <  1:562249/81‑1 (MQ=255)                                                                 aataatGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGc              <  2:562249/80‑1 (MQ=255)                                                                  ataatGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGt            <  2:562250/81‑1 (MQ=255)                                                                        tgAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGc       <  2:562251/80‑1 (MQ=255)                                                                        tgAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGc       <  2:562252/80‑1 (MQ=255)                                                                         gAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGTGATCCAGACTTCGAAGCGTTGAGCaa     >  1:562332/1‑81 (MQ=255)                                                                           gcgcAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGCAAtt   >  1:562317/1‑81 (MQ=255)                                                                            cgcAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGCAATTg  <  1:562254/81‑1 (MQ=255)                                                                               |                                                                              CCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGCAATTG  >  NC_000913/3324506‑3324661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑