Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,328,268	A→G	F150L (TTT→CTT)	greA ←	transcription elongation factor GreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,328,268	0	A	G	100.0%	77.9 / NA	23	F150L (TTT→CTT)	greA	transcription elongation factor GreA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

TTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATA  >  NC_000913/3328193‑3328341                                                                            |                                                                          tttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTc                                                                        <  2:563147/79‑1 (MQ=255) tttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTa                                                                      >  1:563188/1‑81 (MQ=255)    ccTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACtt                                                                   >  2:563183/1‑81 (MQ=255)     ctttctttACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTc                                                                  >  1:563179/1‑81 (MQ=255)         ctttACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTcgccg                                                              >  1:563181/1‑81 (MQ=255)             accaTACATCAACATCTTGAGTATTGGGTAATTCTTTCAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGccc                                                           <  2:563148/77‑1 (MQ=255)             aCAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGccc                                                           <  2:563149/80‑1 (MQ=255)                aTACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCg                                                       >  1:563187/1‑81 (MQ=255)                   cATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGtt                                                     <  2:563150/80‑1 (MQ=255)                    aTCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGtttt                                                   <  2:563151/81‑1 (MQ=255)                            cTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACca                                           >  2:563182/1‑81 (MQ=255)                               gAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACcaca                                         <  2:563152/80‑1 (MQ=255)                                 gTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCacaaca                                      >  1:563190/1‑81 (MQ=255)                                         tAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttc                              <  1:563154/81‑1 (MQ=255)                                          aaTTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttct                             >  2:563184/1‑81 (MQ=255)                                                tACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTg                       >  1:563191/1‑81 (MQ=255)                                                   aGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGcc                     >  1:563194/1‑80 (MQ=255)                                                    ggTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGa                   <  1:563155/81‑1 (MQ=255)                                                     gTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt                  >  1:563192/1‑81 (MQ=255)                                                      tATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATc                 <  1:563157/81‑1 (MQ=255)                                                      tATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATc                 <  1:563156/81‑1 (MQ=255)                                                       aTTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt                  >  1:563193/1‑79 (MQ=255)                                                                      cTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATa  <  1:563158/80‑1 (MQ=255)                                                                            |                                                                          TTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATA  >  NC_000913/3328193‑3328341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑