Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	334,105	T→G	H110P (CAC→CCC)	yahB ←	putative LysR‑type DNA‑binding transcriptional regulator YahB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	334,105	0	T	G	100.0%	94.3 / NA	28	H110P (CAC→CCC)	yahB	putative LysR‑type DNA‑binding transcriptional regulator YahB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/13);  total (15/13)

CGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATT  >  NC_000913/334028‑334179                                                                              |                                                                           cGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGtt                                                                         >  1:69124/1‑81 (MQ=255)         acTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGta                                                                 <  1:69082/81‑1 (MQ=255)                 ggtgACGGCAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAagcag                                                         >  2:69125/1‑81 (MQ=255)                     aCGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTg                                                      <  1:69083/80‑1 (MQ=255)                     aCGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTg                                                      <  2:69084/80‑1 (MQ=255)                     aCGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGa                                                     >  1:69120/1‑81 (MQ=255)                     aCGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGa                                                     >  1:69123/1‑81 (MQ=255)                            tCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATa                                               >  1:69127/1‑80 (MQ=255)                                   ggTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATgcg                                       <  2:69085/81‑1 (MQ=255)                                    gTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATgcg                                       >  1:69130/1‑80 (MQ=255)                                      aGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGca                                     >  1:69117/1‑80 (MQ=255)                                          aaCTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACAcgc                                >  1:69134/1‑81 (MQ=255)                                               cTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCt                             <  1:69086/79‑1 (MQ=255)                                                       gCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCt                   >  1:69122/1‑81 (MQ=255)                                                       gCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCt                   <  2:69087/81‑1 (MQ=255)                                                       gCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCt                   <  2:69088/81‑1 (MQ=255)                                                         aCCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGc                 >  2:69129/1‑81 (MQ=255)                                                         aCCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGc                 <  2:69089/81‑1 (MQ=255)                                                             gTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACATATGCGCACACGCGCTTCTATGCCCTGCGaaa             >  2:69132/1‑81 (MQ=255)                                                             gTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGaaa             >  1:69135/1‑81 (MQ=255)                                                              tAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGaaa             >  1:69119/1‑80 (MQ=255)                                                                aCAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATa           <  1:69090/80‑1 (MQ=255)                                                                  aaGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTa        <  2:69091/81‑1 (MQ=255)                                                                   aGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTAt       >  1:69141/1‑81 (MQ=255)                                                                   aGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTAt       <  2:69092/81‑1 (MQ=255)                                                                       tGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGAt   <  2:69093/81‑1 (MQ=255)                                                                       tGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGAt   <  2:69094/81‑1 (MQ=255)                                                                        gCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGAtt  >  2:69121/1‑81 (MQ=255)                                                                              |                                                                           CGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATT  >  NC_000913/334028‑334179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑