| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,362,620 | T→C | F170S (TTT→TCT) | yhcA → | putative fimbrial chaperone YhcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,362,620 | 0 | T | C | 97.0% | 112.7 / NA | 33 | F170S (TTT→TCT) | yhcA | putative fimbrial chaperone YhcA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (18/14); minor base A (0/1); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACT > NC_000913/3362543‑3362693 | ccTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTa < 1:571375/80‑1 (MQ=255) cTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTaa < 1:571376/80‑1 (MQ=255) ggAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAAc > 1:571414/1‑81 (MQ=255) ggAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAAc > 1:571409/1‑81 (MQ=255) gAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACg < 1:571377/81‑1 (MQ=255) gCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTccc > 1:571407/1‑81 (MQ=255) gCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTccc < 1:571378/81‑1 (MQ=255) gTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCtt < 1:571379/81‑1 (MQ=255) tAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCtt > 1:571404/1‑80 (MQ=255) aaaTGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAg > 1:571415/1‑80 (MQ=255) ggCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGaaa > 1:571421/1‑80 (MQ=255) aaaTTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACggggg > 1:571408/1‑81 (MQ=255) aaTTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGc > 1:571418/1‑81 (MQ=255) aTTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCATACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCt < 1:571380/81‑1 (MQ=255) gagaTTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCtat < 1:571381/80‑1 (MQ=255) ttAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTataaata < 1:571382/81‑1 (MQ=255) ttAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTataaat > 1:571430/1‑80 (MQ=255) tAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTataaat > 1:571410/1‑79 (MQ=255) aaCCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGAcc > 1:571413/1‑81 (MQ=255) aaCCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGAcc < 2:571382/81‑1 (MQ=255) aaCCCCAGTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGAcc > 2:571416/1‑81 (MQ=255) cccAGTAATTATGTCGTTCGTATGAGCTTACAATATAAAACGCGACCTTCAGGAAAAACGGGGGTTATTAATAATACCTAt < 2:571383/81‑1 (MQ=255) gTAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTc < 1:571385/81‑1 (MQ=255) tAATTATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCt < 1:571386/81‑1 (MQ=255) tATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTTTTTTCTACca > 2:571432/1‑81 (MQ=255) tATGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACca > 1:571423/1‑81 (MQ=255) aTGTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACca < 1:571387/80‑1 (MQ=255) gTCGTTCGTATGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACcaca > 1:571419/1‑80 (MQ=255) aTGACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTAc > 1:571422/1‑80 (MQ=255) gACCTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAac > 2:571444/1‑81 (MQ=255) cTTACAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAactac > 2:571430/1‑81 (MQ=255) aCAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAactact < 1:571388/79‑1 (MQ=255) aCAATCTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAactact < 2:571388/79‑1 (MQ=255) | CCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACT > NC_000913/3362543‑3362693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |