Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,371,572	C→T	G335S (GGT→AGT)	nanT ←	N‑acetylneuraminate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,371,572	0	C	T	100.0%	133.7 / NA	37	G335S (GGT→AGT)	nanT	N‑acetylneuraminate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/16);  total (21/16)

CGCAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGT  >  NC_000913/3371495‑3371649                                                                              |                                                                              cGCAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAg                                                                            >  2:573419/1‑81 (MQ=255)  gCAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAg                                                                            <  1:573379/80‑1 (MQ=255)  gCAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAg                                                                            >  2:573423/1‑80 (MQ=255)   cAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAgg                                                                           >  1:573431/1‑80 (MQ=255)   cAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAgg                                                                           >  2:573431/1‑80 (MQ=255)      aTACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGc                                                                       >  1:573426/1‑81 (MQ=255)        aCCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGTGTTCCCAGCCAGTCACTGAGGAAGCCa                                                                     >  1:573435/1‑81 (MQ=255)           ggAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCAcc                                                                   >  2:573427/1‑80 (MQ=255)           ggAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCAcc                                                                   >  1:573430/1‑80 (MQ=255)              ataatCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCTGTCACTGAGGAAGCCACCTACg                                                               >  2:573415/1‑81 (MQ=255)                 atCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACgcag                                                            >  1:573442/1‑81 (MQ=255)                      cagcTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATcc                                                       <  2:573379/81‑1 (MQ=255)                       agcTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATcc                                                       >  2:573426/1‑80 (MQ=255)                       agcTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATcc                                                       >  1:573437/1‑80 (MQ=255)                           gCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCAccg                                                  >  1:573425/1‑81 (MQ=255)                             gAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCAccgcc                                                <  2:573380/81‑1 (MQ=255)                                  cagcagGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCgcgc                                            >  1:573433/1‑80 (MQ=255)                                   agcagGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCa                                          <  1:573382/81‑1 (MQ=255)                                            cAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACt                                  >  2:573445/1‑80 (MQ=255)                                              aaCGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTa                                 >  2:573443/1‑79 (MQ=255)                                               aCGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAg                              <  2:573382/81‑1 (MQ=255)                                                    cGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACa                          <  2:573383/80‑1 (MQ=255)                                                    cGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAg                         >  2:573437/1‑81 (MQ=255)                                                      cTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGca                       <  1:573385/81‑1 (MQ=255)                                                         tGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGcac                      <  2:573385/79‑1 (MQ=255)                                                          gCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACAtt                   >  1:573447/1‑81 (MQ=255)                                                           cGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACAtt                   <  2:573386/80‑1 (MQ=255)                                                           cGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACAtt                   <  2:573387/80‑1 (MQ=255)                                                              gTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCt               <  2:573388/81‑1 (MQ=255)                                                                 cccAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTa              >  1:573450/1‑79 (MQ=255)                                                                 cccAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTa              >  1:573451/1‑79 (MQ=255)                                                                 cccAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTAc             <  1:573390/80‑1 (MQ=255)                                                                  ccagccagTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAg           <  2:573391/81‑1 (MQ=255)                                                                  ccagccagTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAg           <  1:573393/81‑1 (MQ=255)                                                                  ccagccagTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAg           <  1:573391/81‑1 (MQ=255)                                                                         gTCACTGAGGAAGCCACCTACGCAGCATCCCGCCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCgg    <  2:573393/81‑1 (MQ=255)                                                                           cACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGt  >  2:573438/1‑81 (MQ=255)                                                                              |                                                                              CGCAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGT  >  NC_000913/3371495‑3371649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑