Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,375,973	G→A	G369S (GGC→AGC)	dcuD →	putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,375,973	0	G	A	100.0%	131.6 / NA	37	G369S (GGC→AGC)	dcuD	putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/21);  total (16/21)

GGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGC  >  NC_000913/3375897‑3376046                                                                             |                                                                          ggAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGctc                                                                       >  2:574428/1‑81 (MQ=255) ggAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGctc                                                                       >  1:574426/1‑81 (MQ=255)   aGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTg                                                                     >  2:574408/1‑81 (MQ=255)    gCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTgg                                                                    <  2:574352/81‑1 (MQ=255)    gCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTgg                                                                    >  1:574404/1‑81 (MQ=255)     cATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGc                                                                   <  2:574353/81‑1 (MQ=255)           ggtctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATgcg                                                             >  2:574423/1‑81 (MQ=255)            gtctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATgcgc                                                            <  1:574355/81‑1 (MQ=255)             tctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGcc                                                           <  2:574356/81‑1 (MQ=255)             tctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGcc                                                           <  2:574355/81‑1 (MQ=255)                  gCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTa                                                      <  1:574358/81‑1 (MQ=255)                   cGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTAt                                                     >  1:574412/1‑81 (MQ=255)                   cGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTAt                                                     <  1:574359/81‑1 (MQ=255)                    ggTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATg                                                    <  2:574359/81‑1 (MQ=255)                    ggTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATg                                                    <  2:574360/81‑1 (MQ=255)                          ggCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCAttt                                              >  1:574436/1‑81 (MQ=255)                           gCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCAttt                                              <  1:574362/80‑1 (MQ=255)                           gCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTg                                             >  2:574432/1‑81 (MQ=255)                                atgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAg                                         <  1:574363/80‑1 (MQ=255)                                  gatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCt                                      >  2:574430/1‑81 (MQ=255)                                   attattATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCtt                                     <  2:574363/81‑1 (MQ=255)                                       ttatGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTc                                 <  1:574365/81‑1 (MQ=255)                                       ttatGGCGCTGGTCATTGCCATTTGTGCCACTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTc                                 >  2:574427/1‑81 (MQ=255)                                            gcgcTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGaa                             >  1:574427/1‑80 (MQ=255)                                               cTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATc                         <  2:574365/81‑1 (MQ=255)                                                 ggTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCg                        >  1:574423/1‑80 (MQ=255)                                                 ggTCATTGCCATTTGTGCCATCGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCg                        >  2:574418/1‑80 (MQ=255)                                                     aTTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGcc                   >  2:574419/1‑81 (MQ=255)                                                     aTTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGcc                   >  2:574433/1‑81 (MQ=255)                                                         ccATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGAc               >  1:574430/1‑81 (MQ=255)                                                         ccATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTCATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGAc               <  1:574367/81‑1 (MQ=255)                                                               gtgCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACAt          <  1:574368/80‑1 (MQ=255)                                                                tgCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATg         <  1:574369/80‑1 (MQ=255)                                                                tgCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGt        <  2:574370/81‑1 (MQ=255)                                                                tgCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGt        <  2:574369/81‑1 (MQ=255)                                                                    aTTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCa    <  2:574371/81‑1 (MQ=255)                                                                       gtgATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGc  <  1:574373/80‑1 (MQ=255)                                                                             |                                                                          GGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGC  >  NC_000913/3375897‑3376046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑