Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,381,055	C→T	L105L (CTG→TTG)	degQ →	periplasmic serine endoprotease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,381,055	0	C	T	100.0%	114.5 / NA	32	L105L (CTG→TTG)	degQ	periplasmic serine endoprotease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/13);  total (19/13)

ATTTACCGGATCAACCTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGCTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAATGATGGGCGCGAGTTTGATGCAAAA  >  NC_000913/3380978‑3381132                                                                              |                                                                              atTTACCGGATCAACCTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGa                                                                            >  1:575614/1‑81 (MQ=255)    tACCGGATCAACCTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGAcc                                                                          <  2:575564/80‑1 (MQ=255)     aCCGGATCAACCTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACcaa                                                                        >  1:575594/1‑81 (MQ=255)      ccGGATCAACCTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACcaa                                                                        <  2:575565/80‑1 (MQ=255)         gATCAACCTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCaacaac                                                                    >  2:575600/1‑81 (MQ=255)             aaCCTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCAt                                                                 <  1:575567/80‑1 (MQ=255)                cTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGa                                                             >  2:575591/1‑81 (MQ=255)                 tGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGAt                                                            >  1:575613/1‑81 (MQ=255)                   cacaACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGAtt                                                           >  1:575607/1‑80 (MQ=255)                    acaACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTaa                                                         >  2:575611/1‑81 (MQ=255)                      aaCCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAAt                                                        >  2:575596/1‑80 (MQ=255)                           ttCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGca                                                  >  1:575616/1‑81 (MQ=255)                           ttCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGca                                                  >  2:575588/1‑81 (MQ=255)                             cGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGca                                                  >  1:575604/1‑79 (MQ=255)                             cGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGca                                                  <  2:575567/79‑1 (MQ=255)                              gAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGca                                                  >  2:575609/1‑78 (MQ=255)                              gAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAg                                               <  2:575568/81‑1 (MQ=255)                              gAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAg                                               <  2:575570/81‑1 (MQ=255)                              gAAGGTTTAGGCTCCGGTGTCATCATCAACACCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGcaca                                                <  2:575569/80‑1 (MQ=255)                               aaGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGa                                              <  2:575571/81‑1 (MQ=255)                                         cTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTAt                                    >  2:575607/1‑81 (MQ=255)                                           ccGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTAtt                                   >  2:575608/1‑80 (MQ=255)                                            cGGTGTCATCATCAACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTc                                  <  2:575572/80‑1 (MQ=255)                                                 tcatcatcaACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCa                            <  1:575574/81‑1 (MQ=255)                                                   atcatcaACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAat                          >  1:575611/1‑81 (MQ=255)                                                    tcatcaACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAatg                         <  1:575575/81‑1 (MQ=255)                                                    tcatcaACGCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAat                          >  2:575619/1‑80 (MQ=255)                                                            gCCAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAATGATGGgcgc                 >  1:575624/1‑81 (MQ=255)                                                             ccAGTAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAATGATGGgcgc                 >  1:575615/1‑80 (MQ=255)                                                                 tAAAGGCTATGTGTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAATGATGGGCGCGAGtt            <  2:575575/81‑1 (MQ=255)                                                                    aGGCTATGTGTTGACCAACAACCATGAGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAATGATGGGCGCGAGTTTGa         >  2:575617/1‑81 (MQ=255)                                                                          tgtgTTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAATGATGGGCGCGAGTTTGATGCaa    <  2:575576/80‑1 (MQ=255)                                                                           gtgtTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCTGCTCAATGATGGGCGCGAGTTTGATGCaaaa  >  2:575616/1‑81 (MQ=255)                                                                              |                                                                              ATTTACCGGATCAACCTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGCTGACCAACAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAATGATGGGCGCGAGTTTGATGCAAAA  >  NC_000913/3380978‑3381132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑