Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,381,442	G→A	A234T (GCG→ACG)	degQ →	periplasmic serine endoprotease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,381,442	0	G	A	100.0%	115.4 / NA	32	A234T (GCG→ACG)	degQ	periplasmic serine endoprotease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/12);  total (20/12)

CCATTAACCGCGGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTAT  >  NC_000913/3381365‑3381518                                                                              |                                                                             ccATTAACCGCGGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTacgc                                                                           >  1:575673/1‑81 (MQ=255)  cATTAACCGCGGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGcc                                                                          <  1:575642/81‑1 (MQ=255)  cATTAACCGCGGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGcc                                                                          >  2:575683/1‑81 (MQ=255)   aTTAACCGCGGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCt                                                                         >  1:575692/1‑81 (MQ=255)     tAACCGCGGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTgg                                                                       >  2:575684/1‑81 (MQ=255)       aCCGCGGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTggc                                                                      <  2:575642/80‑1 (MQ=255)         cgcgGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTggcggc                                                                   >  2:575696/1‑81 (MQ=255)           cgGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGcggcgg                                                                 <  2:575643/81‑1 (MQ=255)            ggTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGcggcgg                                                                 >  1:575664/1‑80 (MQ=255)            ggTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGcggcgg                                                                 >  1:575698/1‑80 (MQ=255)                 cTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGt                                                           <  1:575645/81‑1 (MQ=255)                 cTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGt                                                           >  1:575705/1‑81 (MQ=255)                  tCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTc                                                          >  2:575677/1‑81 (MQ=255)                          tGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGggattg                                                   >  2:575695/1‑80 (MQ=255)                           gCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGggattgga                                                 >  1:575671/1‑81 (MQ=255)                            cACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGggattggat                                                >  1:575688/1‑81 (MQ=255)                             aCTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGggattggatt                                               >  1:575680/1‑81 (MQ=255)                              cTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGAttt                                              <  2:575645/81‑1 (MQ=255)                                 ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGcc                                           <  1:575647/81‑1 (MQ=255)                                 ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGc                                            >  1:575683/1‑80 (MQ=255)                                    aaCCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATc                                        >  2:575688/1‑81 (MQ=255)                                          aaCGGTGAGTTAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAg                                   >  2:575681/1‑80 (MQ=255)                                                   ttAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTATTATGgcg                         <  2:575647/81‑1 (MQ=255)                                                   ttAATTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcg                         >  1:575701/1‑81 (MQ=255)                                                     aaTTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc                        <  1:575649/80‑1 (MQ=255)                                                      aTTGGCATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGcgcg                       <  2:575649/80‑1 (MQ=255)                                                           cATCAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACt                 <  2:575650/81‑1 (MQ=255)                                                              cAACACTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGgc              >  2:575689/1‑81 (MQ=255)                                                                acacTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGgcg             >  2:575702/1‑80 (MQ=255)                                                                  acTGCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCgcag          <  1:575652/81‑1 (MQ=255)                                                                     gCAATCCTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGcagcag       >  2:575703/1‑81 (MQ=255)                                                                          ccTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTAt  >  1:575695/1‑81 (MQ=255)                                                                           cTTACGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTAt  <  1:575653/80‑1 (MQ=255)                                                                              |                                                                             CCATTAACCGCGGTAACTCCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTAT  >  NC_000913/3381365‑3381518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑