Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,390,209	G→A	L230L (CTG→CTA)	aaeR →	LysR‑type transcriptional regulator AaeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,390,209	0	G	A	100.0%	130.4 / NA	36	L230L (CTG→CTA)	aaeR	LysR‑type transcriptional regulator AaeR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/13);  total (23/13)

ACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGG  >  NC_000913/3390131‑3390279                                                                               |                                                                       aCCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAAc                                                                      >  1:577665/1‑81 (MQ=255)    ggAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAAcggc                                                                   <  2:577615/81‑1 (MQ=255)     ggaGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAAcggcg                                                                  >  1:577670/3‑81 (MQ=255)      aaGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAAcggcgg                                                                 >  2:577662/1‑81 (MQ=255)        gggATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGt                                                               >  2:577661/1‑81 (MQ=255)             ctcGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCgg                                                          >  2:577646/1‑81 (MQ=255)               cGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGa                                                        >  2:577658/1‑81 (MQ=255)                  cTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCg                                                     >  1:577661/1‑81 (MQ=255)                   tCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGc                                                    >  2:577655/1‑81 (MQ=255)                       cTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCtcc                                                >  2:577677/1‑79 (MQ=255)                          aTCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTg                                             >  1:577687/1‑81 (MQ=255)                             ccACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGcc                                           <  1:577617/80‑1 (MQ=255)                                  aGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGAt                                     >  1:577657/1‑81 (MQ=255)                                  aGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCCACGTGCCGCCGAt                                     >  2:577674/1‑81 (MQ=255)                                    gAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGAtgt                                   <  2:577617/81‑1 (MQ=255)                                     aaGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGAtgtg                                  >  2:577683/1‑81 (MQ=255)                                      aGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTgg                                 >  1:577691/1‑81 (MQ=255)                                       gATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTggg                                <  1:577619/81‑1 (MQ=255)                                        aTTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGt                               <  2:577619/81‑1 (MQ=255)                                         tttGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGt                               >  2:577679/1‑80 (MQ=255)                                          ttGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGCTGTGGGTGa                             >  1:577671/1‑81 (MQ=255)                                            gtgACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGAt                            <  1:577621/80‑1 (MQ=255)                                             tgACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATc                           <  2:577621/80‑1 (MQ=255)                                             tgACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGAGTATGGATCGCCTTCGAGCCGCTCATGTGGGTGGTc                           >  2:577668/1‑80 (MQ=255)                                              gACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCaa                         >  1:577673/1‑81 (MQ=255)                                                     gATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCCACGAGAt                   >  2:577680/1‑80 (MQ=255)                                                     gATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGAt                   >  1:577679/1‑80 (MQ=255)                                                      aTCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATc                  <  2:577624/80‑1 (MQ=255)                                                      aTCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCa                 <  2:577622/81‑1 (MQ=255)                                                      aTCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCa                 <  2:577623/81‑1 (MQ=255)                                                         cGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATc              <  1:577626/81‑1 (MQ=255)                                                              aCGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTggg         >  1:577686/1‑81 (MQ=255)                                                              aCGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTggg         >  2:577688/1‑81 (MQ=255)                                                                 cTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAg      <  1:577627/81‑1 (MQ=255)                                                                     tGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTgg  <  1:577628/81‑1 (MQ=255)                                                                      gcgcTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTg   >  1:577683/1‑79 (MQ=255)                                                                               |                                                                       ACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGG  >  NC_000913/3390131‑3390279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑