Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,393,640	T→C	E873E (GAA→GAG)	yhdP ←	AsmA2 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,393,640	0	T	C	100.0%	103.9 / NA	30	E873E (GAA→GAG)	yhdP	AsmA2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/18);  total (12/18)

CACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGT  >  NC_000913/3393564‑3393711                                                                             |                                                                        caccacCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCa                                                                      <  2:578407/80‑1 (MQ=255)     accGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGc                                                                  >  2:578451/1‑80 (MQ=255)        gACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACt                                                              <  1:578409/81‑1 (MQ=255)        gACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACt                                                              >  1:578475/1‑81 (MQ=255)          cacTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACttt                                                            <  1:578411/81‑1 (MQ=255)          cacTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACttt                                                            <  2:578409/81‑1 (MQ=255)            ctctCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGt                                                          <  1:578412/81‑1 (MQ=255)               tcCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGtt                                                         <  1:578413/79‑1 (MQ=255)                 cGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTc                                                        <  1:578414/78‑1 (MQ=255)                 cGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCg                                                      <  2:578414/80‑1 (MQ=255)                       gcCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAAcgg                                               >  1:578469/1‑81 (MQ=255)                         cTTTCTGGAACAGGGCCAGCCACTCGGCACCGTTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAAcggcg                                             <  2:578415/81‑1 (MQ=255)                         cTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAAcggcg                                             >  1:578465/1‑81 (MQ=255)                            tCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAAcggcgg                                            >  1:578474/1‑79 (MQ=255)                                gAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGc                                        <  2:578416/79‑1 (MQ=255)                                gAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGt                                      >  1:578463/1‑81 (MQ=255)                                  aCAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGttt                                    <  2:578417/81‑1 (MQ=255)                                  aCAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGttt                                    <  2:578418/81‑1 (MQ=255)                                     gggCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTAc                                 >  2:578476/1‑81 (MQ=255)                                        ccagccaCTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGt                              >  2:578419/1‑81 (MQ=255)                                             caCTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTgcc                         <  1:578421/81‑1 (MQ=255)                                                  ggCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTgccgcc                      <  2:578422/79‑1 (MQ=255)                                                  ggCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGccc                     <  1:578422/80‑1 (MQ=255)                                                             tCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGAt         >  1:578471/1‑81 (MQ=255)                                                             tCGGCGGCATATTGAGCTCAACGCCACTTTGATCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGAt         <  2:578423/81‑1 (MQ=255)                                                               ggcggcATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATc        <  1:578425/80‑1 (MQ=255)                                                                gcggcATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATc        >  1:578485/1‑79 (MQ=255)                                                                 cggcATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAg     >  2:578469/1‑81 (MQ=255)                                                                  ggcATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGc    >  1:578466/1‑81 (MQ=255)                                                                    cATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGt  <  2:578425/81‑1 (MQ=255)                                                                             |                                                                        CACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGT  >  NC_000913/3393564‑3393711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑