Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,402,941	A→G	F131S (TTC→TCC)	csrD ←	regulator of CsrB and CsrC decay

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,402,941	0	A	G	100.0%	140.6 / NA	40	F131S (TTC→TCC)	csrD	regulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/21);  total (19/21)

CCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGAAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTC  >  NC_000913/3402870‑3403017                                                                        |                                                                             ccGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGc                                                                     <  1:580721/81‑1 (MQ=255)  cGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGcc                                                                    >  1:580757/1‑81 (MQ=255)       ggAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCg                                                               <  2:580721/81‑1 (MQ=255)          agagCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGAt                                                            <  1:580724/81‑1 (MQ=255)          agagCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGAt                                                            <  2:580722/81‑1 (MQ=255)           gagCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATc                                                           <  1:580725/81‑1 (MQ=255)              cATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTg                                                        <  1:580726/81‑1 (MQ=255)                  aCAATGATAAAGCCTATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATa                                                     <  2:580726/80‑1 (MQ=255)                    aaTGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAAc                                                  >  2:580764/1‑81 (MQ=255)                    aaTGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAAc                                                  <  1:580728/81‑1 (MQ=255)                           aaGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACg                                            <  2:580728/80‑1 (MQ=255)                               cAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAAc                                        >  1:580782/1‑80 (MQ=255)                               cAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACg                                       <  1:580731/81‑1 (MQ=255)                               cAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACg                                       <  2:580729/81‑1 (MQ=255)                                 aTCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGa                                      >  2:580773/1‑80 (MQ=255)                                 aTCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGAc                                     <  1:580732/81‑1 (MQ=255)                                    gCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATc                                  >  2:580758/1‑81 (MQ=255)                                    gCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATc                                  >  1:580775/1‑81 (MQ=255)                                    gCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATc                                  >  1:580760/1‑81 (MQ=255)                                      ccccGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATccc                                <  1:580733/81‑1 (MQ=255)                                       cccGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATcccc                               <  1:580735/81‑1 (MQ=255)                                        ccGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCg                              >  1:580777/1‑81 (MQ=255)                                        ccGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCg                              >  2:580780/1‑81 (MQ=255)                                          gTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGa                            >  1:580772/1‑81 (MQ=255)                                             aGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGc                         <  1:580736/81‑1 (MQ=255)                                              gcggcgCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCt                        <  1:580737/81‑1 (MQ=255)                                                 gcgcGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTa                     >  1:580787/1‑81 (MQ=255)                                                   gcgGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTAt                    >  1:580780/1‑80 (MQ=255)                                                     ggtggtCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCaa                 >  2:580791/1‑81 (MQ=255)                                                      gtggtCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAAc                <  1:580738/81‑1 (MQ=255)                                                        ggtCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACg               >  2:580778/1‑80 (MQ=255)                                                         gtCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGa             <  2:580738/81‑1 (MQ=255)                                                            atcaACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAAc           <  2:580739/80‑1 (MQ=255)                                                              caACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCt        >  1:580785/1‑81 (MQ=255)                                                              caACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCt        >  2:580770/1‑81 (MQ=255)                                                              caACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCt        >  2:580775/1‑81 (MQ=255)                                                               aaCGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTc       <  1:580741/81‑1 (MQ=255)                                                                   aaTGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGtt   >  2:580788/1‑81 (MQ=255)                                                                    aTGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTc  >  1:580766/1‑81 (MQ=255)                                                                    aTGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTc  <  1:580742/81‑1 (MQ=255)                                                                        |                                                                             CCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGAAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTC  >  NC_000913/3402870‑3403017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑