Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,411,849	T→C	F66S (TTT→TCT)	yhdJ →	DNA adenine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,849	0	T	C	100.0%	94.6 / NA	28	F66S (TTT→TCT)	yhdJ	DNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/8);  total (20/8)

ATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAA  >  NC_000913/3411785‑3411919                                                                 |                                                                       aTCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCagag                                                        <  1:582832/81‑1 (MQ=255) aTCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCagag                                                        >  1:582878/1‑81 (MQ=255)      tAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCa                                                   <  2:582833/81‑1 (MQ=255)      tAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCa                                                   <  2:582832/81‑1 (MQ=255)        aaaaTTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCAcc                                                 <  1:582835/81‑1 (MQ=255)            ttttGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGt                                             >  1:582879/1‑81 (MQ=255)                 aTGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTg                                         >  2:582874/1‑80 (MQ=255)                 aTGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTg                                         <  2:582835/80‑1 (MQ=255)                   ggTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGaa                                       >  1:582896/1‑80 (MQ=255)                       tGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAg                                   >  2:582871/1‑80 (MQ=255)                         aTCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGc                                  <  2:582836/79‑1 (MQ=255)                           cGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                              >  2:582880/1‑81 (MQ=255)                           cGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                              >  1:582885/1‑81 (MQ=255)                            gAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                              >  2:582894/1‑80 (MQ=255)                                   ggAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATg                       >  1:582901/1‑80 (MQ=255)                                    gAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTa                     <  2:582837/81‑1 (MQ=255)                                     aaaGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTa                     >  1:582899/1‑80 (MQ=255)                                     aaaGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTa                     >  1:582877/1‑80 (MQ=255)                                     aaaGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTa                     >  2:582886/1‑80 (MQ=255)                                      aagaagATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAca                   >  1:582894/1‑81 (MQ=255)                                         aagaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAcatca                >  2:582883/1‑81 (MQ=255)                                           gaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAcatcat               >  2:582887/1‑80 (MQ=255)                                              cTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGaa            >  1:582897/1‑80 (MQ=255)                                              cTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGaa            >  1:582889/1‑80 (MQ=255)                                                 tttATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGaa            <  1:582839/77‑1 (MQ=255)                                                    aTCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACg     >  1:582902/1‑81 (MQ=255)                                                    aTCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACg     >  1:582890/1‑81 (MQ=255)                                                        aCTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGaa  >  1:582900/1‑80 (MQ=255)                                                                 |                                                                       ATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAA  >  NC_000913/3411785‑3411919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑