Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,431,553	G→A	A137V (GCC→GTC)	yrdD ←	putative DNA topoisomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,431,553	0	G	A	100.0%	83.2 / NA	24	A137V (GCC→GTC)	yrdD	putative DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/15);  total (9/15)

CGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGC  >  NC_000913/3431480‑3431628                                                                          |                                                                            cgcgGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTg                                                                      <  2:587300/81‑1 (MQ=255) agcgGTTTTCTTTTCGATGAGTAGAGGATAATGACAATCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTg                                                                      <  1:587302/80‑1 (MQ=255)       tttcttttcGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTc                                                                >  2:587348/1‑81 (MQ=255)        ttcttttcGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTc                                                                <  1:587303/80‑1 (MQ=255)           ttttcGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCggg                                                            >  1:587352/1‑81 (MQ=255)              tcGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTa                                                          <  2:587303/80‑1 (MQ=255)               cGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGc                                                        >  1:587359/1‑81 (MQ=255)                  tGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGAt                                                     <  2:587304/81‑1 (MQ=255)                  tGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGAt                                                     <  2:587305/81‑1 (MQ=255)                    aGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATc                                                    <  1:587307/80‑1 (MQ=255)                       aGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATcac                                                  >  1:587358/1‑79 (MQ=255)                        gCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATcac                                                  >  2:587350/1‑78 (MQ=255)                        gCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACaa                                                <  2:587307/80‑1 (MQ=255)                         cGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAaga                                              <  2:587308/81‑1 (MQ=255)                              aaTGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGa                                          >  2:587352/1‑80 (MQ=255)                                  acacTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGt                                     >  1:587356/1‑81 (MQ=255)                                    acTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGttt                                   >  2:587349/1‑81 (MQ=255)                                       cAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGCGTGAAATGTTTTGc                                >  2:587341/1‑81 (MQ=255)                                        aGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGc                                <  1:587310/80‑1 (MQ=255)                                          ggCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCAt                             <  2:587310/81‑1 (MQ=255)                                                   cAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGa                      <  1:587312/79‑1 (MQ=255)                                                   cAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAgc                    <  1:587313/81‑1 (MQ=255)                                                         tGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAgcggcg                <  2:587313/79‑1 (MQ=255)                                                                      aaTGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGc  <  1:587315/80‑1 (MQ=255)                                                                          |                                                                            CGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGC  >  NC_000913/3431480‑3431628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑