Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,467,731	G→A	A708V (GCA→GTA)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,467,731	0	G	A	100.0%	125.6 / NA	35	A708V (GCA→GTA)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/17);  total (18/17)

CAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAAT  >  NC_000913/3467653‑3467806                                                                               |                                                                            cAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATAcc                                                                           <  1:595925/81‑1 (MQ=255) cAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATAcc                                                                           >  2:595991/1‑81 (MQ=255) cAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATAcc                                                                           >  2:595972/1‑81 (MQ=255)  aGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCt                                                                          <  2:595925/81‑1 (MQ=255)  aGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCt                                                                          >  1:595975/1‑81 (MQ=255)    aTAGCAATATCTTTGCCTTCTGACTCCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGa                                                                        <  1:595927/81‑1 (MQ=255)      aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATc                                                                      >  2:595959/1‑81 (MQ=255)        cAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCg                                                                    >  1:595988/1‑81 (MQ=255)          atatCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGc                                                                   >  2:595969/1‑80 (MQ=255)                 tGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAAccc                                                              <  2:595927/78‑1 (MQ=255)                  gCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGt                                                          >  2:595963/1‑81 (MQ=255)                    cTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGc                                                        >  2:595966/1‑81 (MQ=255)                       cTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCt                                                      <  1:595929/80‑1 (MQ=255)                         gACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTc                                                    >  1:595968/1‑80 (MQ=255)                          aCTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTc                                                    <  2:595929/79‑1 (MQ=255)                          aCTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGa                                                  >  1:595976/1‑81 (MQ=255)                               cATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATc                                              >  1:595974/1‑80 (MQ=255)                                     tCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTcc                                       <  1:595931/81‑1 (MQ=255)                                     tCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTcc                                       <  2:595932/81‑1 (MQ=255)                                     tCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTc                                        <  2:595931/80‑1 (MQ=255)                                          cACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGAc                                  <  2:595934/81‑1 (MQ=255)                                          cACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGAc                                  <  2:595933/81‑1 (MQ=255)                                            cTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACtt                                >  2:595981/1‑81 (MQ=255)                                             tttCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACttt                               >  2:595979/1‑81 (MQ=255)                                                   cacaGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTACCTTCGCTATCGAAGTCCAGGACTTTGAGGtt                         >  2:595984/1‑81 (MQ=255)                                                     caGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAAGCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCa                       <  1:595937/81‑1 (MQ=255)                                                     caGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCa                       <  1:595936/81‑1 (MQ=255)                                                             ttACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCa               <  1:595938/81‑1 (MQ=255)                                                                cgacgTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAAc             >  2:595938/1‑80 (MQ=255)                                                                   cgTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATg         <  2:595939/81‑1 (MQ=255)                                                                    gTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGt        <  2:595940/81‑1 (MQ=255)                                                                    gTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGt        >  2:595988/1‑81 (MQ=255)                                                                        aaTAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCat     >  1:595987/1‑80 (MQ=255)                                                                         aTAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCataa   <  1:595942/81‑1 (MQ=255)                                                                           aGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCataat  >  2:595985/1‑80 (MQ=255)                                                                               |                                                                            CAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAAT  >  NC_000913/3467653‑3467806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑