Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,469,803	A→G	C17C (TGT→TGC)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,469,803	0	A	G	100.0%	55.5 / NA	17	C17C (TGT→TGC)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/12);  total (5/12)

CATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACG  >  NC_000913/3469727‑3469870                                                                             |                                                                    cATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAgcaca                                                                 <  2:596401/81‑1 (MQ=255)   tCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACAcc                                                               <  2:596402/81‑1 (MQ=255)        aaTAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGccc                                                          >  2:596430/1‑81 (MQ=255)         aTAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGcccc                                                         <  2:596403/81‑1 (MQ=255)            aCCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCAt                                                       <  2:596404/80‑1 (MQ=255)              cTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATAc                                                     >  1:596425/1‑80 (MQ=255)                    tttattACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCAt                                              <  2:596405/81‑1 (MQ=255)                    tttattACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCAt                                              <  2:596406/81‑1 (MQ=255)                                             cATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTc                     <  1:596408/81‑1 (MQ=255)                                              aTGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCa                    >  1:596430/1‑81 (MQ=255)                                              aTGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCa                    <  2:596408/81‑1 (MQ=255)                                                 cTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCAtt                  <  1:596411/80‑1 (MQ=255)                                                 cTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCAtt                  <  2:596409/80‑1 (MQ=255)                                                         gCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCt         >  2:596428/1‑81 (MQ=255)                                                          ccAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCtt        <  1:596412/81‑1 (MQ=255)                                                            aaTGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTtgt      <  1:596413/81‑1 (MQ=255)                                                                cTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACg  <  2:596413/81‑1 (MQ=255)                                                                 tGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACg  >  2:596433/1‑80 (MQ=255)                                                                             |                                                                    CATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACG  >  NC_000913/3469727‑3469870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑