Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,702	A→T	F605I (TTT→ATT)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,702	0	A	T	100.0%	116.1 / NA	32	F605I (TTT→ATT)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/16);  total (16/16)

GGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCG  >  NC_000913/3471631‑3471775                                                                        |                                                                          ggTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCcttctt                                                                   >  1:596859/1‑80 (MQ=255)            ccGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGa                                                          <  1:596822/78‑1 (MQ=255)             cGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAg                                                       <  2:596822/80‑1 (MQ=255)               gAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAag                                                    >  2:596859/1‑81 (MQ=255)                  tttCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAagcag                                                 <  2:596823/81‑1 (MQ=255)                      tACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAg                                             >  1:596873/1‑81 (MQ=255)                        cTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGtt                                           >  1:596865/1‑81 (MQ=255)                        cTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGt                                            <  1:596826/80‑1 (MQ=255)                        cTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGt                                            <  2:596824/80‑1 (MQ=255)                           cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTa                                         >  2:596862/1‑80 (MQ=255)                           cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTa                                         <  1:596827/80‑1 (MQ=255)                                 tCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGc                                    >  1:596869/1‑79 (MQ=255)                                  cATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAg                                 >  1:596866/1‑81 (MQ=255)                                    tGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt                               <  2:596829/81‑1 (MQ=255)                                    tGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt                               <  2:596827/81‑1 (MQ=255)                                    tGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt                               <  1:596829/81‑1 (MQ=255)                                       tCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt                               >  2:596866/1‑78 (MQ=255)                                         ggCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag                          <  1:596831/81‑1 (MQ=255)                                            tCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAgaggag                       >  2:596873/1‑81 (MQ=255)                                             cAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGt                      >  2:596901/1‑81 (MQ=255)                                              aaGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGt                      >  2:596867/1‑80 (MQ=255)                                              aaGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTc                     <  2:596831/81‑1 (MQ=255)                                               aGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCa                    <  2:596832/81‑1 (MQ=255)                                                gCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCaa                   >  1:596876/1‑81 (MQ=255)                                                  aGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACg                 <  2:596833/81‑1 (MQ=255)                                                        ggTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATgg           >  1:596887/1‑81 (MQ=255)                                                        ggTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATgg           >  2:596889/1‑81 (MQ=255)                                                         gTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATg            <  2:596834/79‑1 (MQ=255)                                                              gCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGaa     >  1:596878/1‑81 (MQ=255)                                                                tttCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAAcc   >  1:596870/1‑81 (MQ=255)                                                                  tCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAAcc   <  1:596836/79‑1 (MQ=255)                                                                  tCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg  <  1:596837/80‑1 (MQ=255)                                                                        |                                                                          GGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCG  >  NC_000913/3471631‑3471775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑