Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,485,644	C→T	G59R (GGG→AGG)	yhfA ←	OsmC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,485,644	0	C	T	100.0%	110.1 / NA	31	G59R (GGG→AGG)	yhfA	OsmC family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/24);  total (7/24)

TGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTG  >  NC_000913/3485566‑3485721                                                                               |                                                                              tgtgCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCttt                                                                             <  1:600246/81‑1 (MQ=255) tgtgCGT‑AACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCtttt                                                                            <  2:600246/81‑1 (MQ=255)  gtgCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCtttt                                                                            >  2:600325/1‑81 (MQ=255)     cGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCtttttt                                                                          <  2:600247/80‑1 (MQ=255)      gTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTg                                                                         >  2:600306/1‑80 (MQ=255)         aaCAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAgg                                                                     <  1:600249/81‑1 (MQ=255)          aCAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGa                                                                    <  2:600249/81‑1 (MQ=255)           cAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGAt                                                                   <  2:600250/81‑1 (MQ=255)            aGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATc                                                                  <  1:600252/81‑1 (MQ=255)               cGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGaa                                                               <  1:600253/81‑1 (MQ=255)                gtggtgCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGaaa                                                              >  2:600299/1‑81 (MQ=255)                 tggtgCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAAc                                                             <  2:600253/81‑1 (MQ=255)                      ccTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACcaca                                                         <  2:600254/80‑1 (MQ=255)                       ctctTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATc                                                       <  1:600256/81‑1 (MQ=255)                       ctctTCGCGGCGTTCAGAGGTCAATTTTACTACACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATc                                                       <  2:600256/81‑1 (MQ=255)                          ttCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCg                                                      <  2:600257/79‑1 (MQ=255)                          ttCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGAt                                                    >  1:600301/1‑81 (MQ=255)                           tCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATg                                                   >  2:600298/1‑81 (MQ=255)                            cgcgGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATg                                                   <  1:600259/80‑1 (MQ=255)                                   ttCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCa                                           <  2:600259/81‑1 (MQ=255)                                    tCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCaa                                          >  2:600313/1‑81 (MQ=255)                                          gTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCAccc                                    <  2:600260/81‑1 (MQ=255)                                          gTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCAccc                                    <  1:600263/81‑1 (MQ=255)                                          gTCAATTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCAccc                                    <  1:600262/81‑1 (MQ=255)                                             aaTTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACccgcc                                 <  1:600264/81‑1 (MQ=255)                                              aTTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACccgccg                                <  2:600264/81‑1 (MQ=255)                                              aTTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACccgccg                                <  1:600266/81‑1 (MQ=255)                                              aTTTTACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACccgccg                                >  2:600311/1‑81 (MQ=255)                                                 ttACTTCACAATCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCgccgcc                              <  2:600266/80‑1 (MQ=255)                                                           aTCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCAt                   <  2:600268/81‑1 (MQ=255)                                                           aTCGACCACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGAACTGCAACCACCCGCCGCCATCAACACCAt                   <  2:600267/81‑1 (MQ=255)                                                                ccACATCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCa              >  1:600313/1‑81 (MQ=255)                                                                    aTCCTGACGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCgg          <  1:600270/81‑1 (MQ=255)                                                                          aCGCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACtt      <  1:600271/79‑1 (MQ=255)                                                                            gCCTTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTg  <  1:600272/81‑1 (MQ=255)                                                                               |                                                                              TGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTG  >  NC_000913/3485566‑3485721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑