Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	342,381	A→C	K419N (AAA→AAC)	yahJ →	putative deaminase with metallo‑dependent hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	342,381	0	A	C	100.0%	88.9 / NA	26	K419N (AAA→AAC)	yahJ	putative deaminase with metallo‑dependent hydrolase domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/15);  total (11/15)

TCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAAC  >  NC_000913/342310‑342458                                                                        |                                                                              tcCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGTGCGTGTATGGCCAAACGCGCAg                                                                         <  2:70794/78‑1 (MQ=255)   ccGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGa                                                                       <  2:70795/78‑1 (MQ=255)    cgctcgctGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGAc                                                                   <  2:70796/81‑1 (MQ=255)         gctGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGcc                                                                <  2:70797/79‑1 (MQ=255)          ctGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGc                                                             <  1:70798/81‑1 (MQ=255)           tGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCt                                                            >  2:70846/1‑81 (MQ=255)            gTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCtt                                                           >  2:70834/1‑81 (MQ=255)                ttAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTtgt                                                        <  2:70799/80‑1 (MQ=255)                          gCGATGTATTGCCGCTGAATGAAAAGGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGAc                                              <  2:70801/80‑1 (MQ=255)                          gCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGAc                                              <  1:70800/80‑1 (MQ=255)                          gCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACg                                             >  2:70853/1‑81 (MQ=255)                           cGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGc                                            >  1:70849/1‑81 (MQ=255)                            gATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGcc                                           >  1:70843/1‑81 (MQ=255)                              tGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGcctc                                         >  1:70839/1‑81 (MQ=255)                                   tGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGtt                                    >  1:70848/1‑81 (MQ=255)                                           aatgaaAAAGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAg                            <  2:70803/81‑1 (MQ=255)                                           aatgaaAAAGGCGAACGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAg                            <  2:70802/81‑1 (MQ=255)                                               aaaaaGGCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCg                         <  2:70804/80‑1 (MQ=255)                                                     gCGAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGgcgc                  <  2:70805/81‑1 (MQ=255)                                                       gAGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGcgcg                 >  1:70855/1‑80 (MQ=255)                                                        aGCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTa               >  1:70840/1‑81 (MQ=255)                                                         gCGTGTATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTAt              <  1:70806/81‑1 (MQ=255)                                                           gtgtATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTAtct            >  2:70860/1‑81 (MQ=255)                                                            tgtATGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTAtctc           <  2:70807/81‑1 (MQ=255)                                                               aTGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGcc        >  2:70845/1‑81 (MQ=255)                                                                tGGCCAAACGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCg       <  1:70808/81‑1 (MQ=255)                                                                     aaacGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAAc  <  1:70809/81‑1 (MQ=255)                                                                        |                                                                              TCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAAC  >  NC_000913/342310‑342458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑