Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,497,365	G→A	M121I (ATG→ATA)	nirC →	nitrite transporter NirC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,497,365	0	G	A	100.0%	125.5 / NA	35	M121I (ATG→ATA)	nirC	nitrite transporter NirC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/17);  total (18/17)

ATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGCTAA  >  NC_000913/3497288‑3497436                                                                              |                                                                        aTCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATActc                                                                      >  2:603189/1‑81 (MQ=255)  tCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATActct                                                                     <  2:603147/81‑1 (MQ=255)   cAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCta                                                                    >  2:603203/1‑81 (MQ=255)    aGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCta                                                                    <  2:603148/80‑1 (MQ=255)         cGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTg                                                              >  1:603207/1‑81 (MQ=255)          gggCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTgg                                                             >  1:603212/1‑81 (MQ=255)           ggCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTggg                                                            <  1:603150/81‑1 (MQ=255)              aaaTGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGggcg                                                         >  1:603206/1‑81 (MQ=255)               aaTGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGggcgg                                                        >  1:603209/1‑81 (MQ=255)               aaTGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGggcgg                                                        >  1:603221/1‑81 (MQ=255)               aaTGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGggcgg                                                        >  2:603216/1‑81 (MQ=255)                  gtgGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGcggcgg                                                     >  1:603223/1‑81 (MQ=255)                   tgGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGcggcgg                                                     <  2:603152/80‑1 (MQ=255)                   tgGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGt                                                    <  2:603151/81‑1 (MQ=255)                   tgGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGt                                                    <  2:603150/81‑1 (MQ=255)                        aaTCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCct                                               >  2:603213/1‑81 (MQ=255)                               ccGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCg                                        <  1:603154/81‑1 (MQ=255)                                 gCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCgg                                       >  2:603219/1‑80 (MQ=255)                                 gCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGt                                      <  2:603154/81‑1 (MQ=255)                                 gCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGt                                      <  1:603156/81‑1 (MQ=255)                                  cAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTa                                     <  2:603156/81‑1 (MQ=255)                                      ccTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATa                                 <  2:603157/81‑1 (MQ=255)                                             gggTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCAt                          >  1:603208/1‑81 (MQ=255)                                              ggTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATc                         <  1:603159/81‑1 (MQ=255)                                                 aaCCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGtt                      >  1:603218/1‑81 (MQ=255)                                                      ggTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTc                 >  2:603210/1‑81 (MQ=255)                                                      ggTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTc                 >  1:603222/1‑81 (MQ=255)                                                       gTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCGCTcc                <  2:603159/81‑1 (MQ=255)                                                          ggTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTc             <  2:603160/81‑1 (MQ=255)                                                               cGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCTTCGCGCt        <  2:603161/81‑1 (MQ=255)                                                               cGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCgcgc         <  1:603163/80‑1 (MQ=255)                                                                  cTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGc     >  2:603214/1‑81 (MQ=255)                                                                  cTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGc     >  2:603266/1‑81 (MQ=255)                                                                     cGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGc     <  2:603163/78‑1 (MQ=255)                                                                      gTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGCTaa  >  2:603212/1‑80 (MQ=255)                                                                              |                                                                        ATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGCTAA  >  NC_000913/3497288‑3497436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑