Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,500,149	G→A	L80L (CTG→CTA)	frlA →	fructoselysine/psicoselysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,500,149	0	G	A	100.0%	124.0 / NA	34	L80L (CTG→CTA)	frlA	fructoselysine/psicoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

ATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGT  >  NC_000913/3500072‑3500225                                                                              |                                                                             aTTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTaaaa                                                                           <  1:603728/81‑1 (MQ=255)     tgATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAAt                                                                        <  2:603728/80‑1 (MQ=255)      gATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGc                                                                      >  2:603788/1‑81 (MQ=255)          ccGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGa                                                                  <  1:603730/81‑1 (MQ=255)              aaaTGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGcc                                                              <  1:603731/81‑1 (MQ=255)               aaTGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCg                                                             <  1:603732/81‑1 (MQ=255)               aaTGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCg                                                             <  2:603732/81‑1 (MQ=255)                  gtgCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGAcc                                                          >  1:603781/1‑81 (MQ=255)                   tgCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCg                                                         <  1:603734/81‑1 (MQ=255)                   tgCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCg                                                         <  1:603735/81‑1 (MQ=255)                     cGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCt                                                       <  1:603736/81‑1 (MQ=255)                            gCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTc                                                >  1:603784/1‑81 (MQ=255)                              ggAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCct                                              <  2:603736/81‑1 (MQ=255)                              ggAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCct                                              >  1:603785/1‑81 (MQ=255)                               gAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCctc                                             <  1:603738/81‑1 (MQ=255)                                        aCCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTgg                                    >  2:603798/1‑81 (MQ=255)                                        aCCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTgg                                    >  1:603804/1‑81 (MQ=255)                                        aCCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTgg                                    >  1:603792/1‑81 (MQ=255)                                               aTCCGGAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAg                               <  1:603739/79‑1 (MQ=255)                                                   ggAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTg                         >  2:603809/1‑81 (MQ=255)                                                    gAAAATGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTgg                        >  2:603786/1‑81 (MQ=255)                                                     aaaaTGGCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTggg                       <  1:603740/81‑1 (MQ=255)                                                          ggCGCAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAAc                  >  2:603787/1‑81 (MQ=255)                                                           gcgcAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAAc                  <  2:603740/80‑1 (MQ=255)                                                            cgcAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGa                >  2:603799/1‑81 (MQ=255)                                                             gcAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGAt               <  2:603741/81‑1 (MQ=255)                                                              cAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATg              >  2:603785/1‑81 (MQ=255)                                                              cAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATg              <  2:603742/81‑1 (MQ=255)                                                              cAGATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATg              >  1:603807/1‑81 (MQ=255)                                                                gATTATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATgcg            >  2:603795/1‑81 (MQ=255)                                                                  ttATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGcc          <  1:603744/81‑1 (MQ=255)                                                                   tATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCg         >  1:603795/1‑81 (MQ=255)                                                                   tATGTTTATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCg         >  2:603801/1‑81 (MQ=255)                                                                        ttATCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCAtt    >  1:603808/1‑81 (MQ=255)                                                                           tCTAAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGt  <  1:603745/80‑1 (MQ=255)                                                                              |                                                                             ATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGT  >  NC_000913/3500072‑3500225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑