Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,545,060	C→A	P60H (CCC→CAC)	yhgH →	DNA utilization protein YhgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,545,060	0	C	A	100.0%	125.0 / NA	35	P60H (CCC→CAC)	yhgH	DNA utilization protein YhgH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/19);  total (16/19)

AACGTTATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGC  >  NC_000913/3544983‑3545137                                                                              |                                                                              aaCGTTATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTg                                                                            <  1:615308/81‑1 (MQ=255)   cGTTATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGc                                                                          <  2:615308/81‑1 (MQ=255)    gTTATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCa                                                                         <  2:615309/81‑1 (MQ=255)     ttATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCa                                                                         >  2:615368/1‑80 (MQ=255)        tGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAg                                                                      <  2:615310/80‑1 (MQ=255)              caATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGt                                                                >  1:615360/1‑80 (MQ=255)               aaTGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCa                                                              <  1:615312/81‑1 (MQ=255)               aaTGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCa                                                              >  2:615347/1‑81 (MQ=255)                       ttACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGcc                                                      >  2:615370/1‑81 (MQ=255)                         aCCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCg                                                     <  2:615312/80‑1 (MQ=255)                         aCCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGa                                                    >  1:615367/1‑81 (MQ=255)                           ccGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACt                                                  <  2:615313/81‑1 (MQ=255)                             gCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTAt                                                >  1:615358/1‑81 (MQ=255)                               cacacaCTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATgc                                              <  2:615314/81‑1 (MQ=255)                                acacacTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATgcg                                             >  2:615365/1‑81 (MQ=255)                                acacacTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATgcg                                             <  1:615316/81‑1 (MQ=255)                                    acTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGcgccgc                                         <  2:615316/81‑1 (MQ=255)                                     cTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCgccgcc                                        <  2:615317/81‑1 (MQ=255)                                     cTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCgccgcc                                        <  1:615319/81‑1 (MQ=255)                                        ccATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGtt                                     <  1:615320/81‑1 (MQ=255)                                         cATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGtt                                     >  1:615373/1‑80 (MQ=255)                                          aTCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGtt                                     >  2:615376/1‑79 (MQ=255)                                               cccTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTc                                <  1:615321/79‑1 (MQ=255)                                               cccTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCg                              >  1:615364/1‑81 (MQ=255)                                                 cTGCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCt                            >  1:615322/1‑81 (MQ=255)                                                   gCGGTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTa                          >  2:615364/1‑81 (MQ=255)                                                     ggTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATc                        >  1:615370/1‑81 (MQ=255)                                                      gTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATcc                       <  1:615323/81‑1 (MQ=255)                                                      gTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATcc                       <  1:615324/81‑1 (MQ=255)                                                      gTCGCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATc                        >  2:615371/1‑80 (MQ=255)                                                         gCTGCCTGCAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATccac                     >  1:615368/1‑80 (MQ=255)                                                           tgcctgcAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAg                  >  2:615378/1‑81 (MQ=255)                                                                 cAAAAACCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTaaa            <  2:615324/81‑1 (MQ=255)                                                                    aaaCCGCCGCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAAttt         <  2:615325/81‑1 (MQ=255)                                                                       ccgccgCACTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTcc      <  1:615327/81‑1 (MQ=255)                                                                         gccgcaCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCg    <  2:615328/81‑1 (MQ=255)                                                                         gccgcaCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCg    <  1:615328/81‑1 (MQ=255)                                                                           cgcaCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGgc  <  1:615330/81‑1 (MQ=255)                                                                              |                                                                              AACGTTATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGC  >  NC_000913/3544983‑3545137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑