Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,568,321	G→A	G336G (GGC→GGT)	glgC ←	glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,568,321	0	G	A	100.0%	120.0 / NA	35	G336G (GGC→GGT)	glgC	glucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/19);  total (16/19)

TGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGATT  >  NC_000913/3568245‑3568397                                                                             |                                                                             tGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccaccg                                                                           >  1:621431/1‑80 (MQ=255)  gTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCgg                                                                          >  1:621415/1‑80 (MQ=255)   ttGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGa                                                                         <  2:621349/80‑1 (MQ=255)   ttGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGa                                                                         <  1:621351/80‑1 (MQ=255)     gCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAAc                                                                      >  1:621416/1‑81 (MQ=255)         aatgaatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGt                                                                  >  1:621436/1‑81 (MQ=255)             aatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGt                                                              >  2:621421/1‑81 (MQ=255)              atTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGtt                                                             <  1:621352/81‑1 (MQ=255)               ttCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTa                                                            <  1:621353/81‑1 (MQ=255)                    gcgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGt                                                       >  1:621428/1‑81 (MQ=255)                    gcgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGt                                                       <  2:621353/81‑1 (MQ=255)                      gAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCa                                                     <  1:621355/81‑1 (MQ=255)                      gAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCa                                                     >  2:621413/1‑81 (MQ=255)                      gAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCa                                                     >  1:621420/1‑81 (MQ=255)                      gAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCa                                                     >  1:621426/1‑81 (MQ=255)                       aaCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCAt                                                    <  1:621356/81‑1 (MQ=255)                         cgcgcgAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATcc                                                  <  1:621357/81‑1 (MQ=255)                              gagaACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTgg                                             >  1:621417/1‑81 (MQ=255)                              gagaACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTgg                                             >  2:621411/1‑81 (MQ=255)                                gaacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc                                            >  1:621418/1‑80 (MQ=255)                                gaacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCt                                           <  1:621358/81‑1 (MQ=255)                                 aacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTa                                          <  2:621358/81‑1 (MQ=255)                                     gaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCgg                                      <  2:621359/81‑1 (MQ=255)                                            cTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATc                               <  1:621361/81‑1 (MQ=255)                                               caccaccacCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTg                            >  1:621442/1‑81 (MQ=255)                                                accaccaccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGc                           <  2:621361/81‑1 (MQ=255)                                                      accGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAAt                     <  2:621362/81‑1 (MQ=255)                                                        cGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAAttt                   >  2:621437/1‑81 (MQ=255)                                                         gAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTc                  >  1:621425/1‑81 (MQ=255)                                                              ggAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTgg             <  1:621364/81‑1 (MQ=255)                                                               gagaTCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGc            <  2:621364/81‑1 (MQ=255)                                                                agaTCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCg           <  2:621365/81‑1 (MQ=255)                                                                     acacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAAt      <  1:621367/81‑1 (MQ=255)                                                                      cacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATg     <  2:621367/81‑1 (MQ=255)                                                                       acaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGa    >  2:621429/1‑81 (MQ=255)                                                                         aaCCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGAtt  >  1:621423/1‑81 (MQ=255)                                                                             |                                                                             TGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGATT  >  NC_000913/3568245‑3568397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑