Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,577,424	G→A	L57L (CTG→TTG)	gntK ←	D‑gluconate kinase, thermostable

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,577,424	0	G	A	100.0%	100.5 / NA	28	L57L (CTG→TTG)	gntK	D‑gluconate kinase, thermostable
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/15);  total (13/15)

TATTAGTGCGCTGCATAGCAAACGCGGCGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAGTGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAACGCGGCATG  >  NC_000913/3577352‑3577496                                                                         |                                                                         tattaGTGCGCTGCATAGCAAACGCGGCGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCg                                                                   <  1:623745/80‑1 (MQ=255)                 aGCAAACGCGGCGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTc                                                  >  1:623808/1‑81 (MQ=255)                 aGCAAACGCGGCGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTc                                                  >  1:623797/1‑81 (MQ=255)                  gCAAACGCGGCGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTc                                                  <  2:623745/80‑1 (MQ=255)                  gCAAACGCGGCGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTc                                                  >  1:623801/1‑80 (MQ=255)                  gCAAACGCGGAGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACACCATTTTTTCg                                                 <  2:623746/81‑1 (MQ=255)                      aCGCGGCGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGatat                                             >  2:623795/1‑81 (MQ=255)                       cgcgGCGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATAtt                                            >  1:623795/1‑81 (MQ=255)                         cggcgTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTgc                                          <  1:623748/81‑1 (MQ=255)                         cggcgTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTgc                                          <  2:623748/81‑1 (MQ=255)                          ggcgTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTgcg                                         >  1:623799/1‑81 (MQ=255)                             gtcgtTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCg                                      >  2:623808/1‑81 (MQ=255)                                gtTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGtg                                    >  2:623792/1‑80 (MQ=255)                                   cAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGtggatg                                >  1:623806/1‑81 (MQ=255)                                            cAACCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGaaa                         <  1:623750/79‑1 (MQ=255)                                             aaCCACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCg                      >  2:623803/1‑81 (MQ=255)                                               ccACGGTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGcc                    <  1:623751/81‑1 (MQ=255)                                                    gTTTGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCaa               <  2:623751/81‑1 (MQ=255)                                                     tttGCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAg              <  2:623752/81‑1 (MQ=255)                                                        gCGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGaa            <  2:623753/80‑1 (MQ=255)                                                         cGATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAAc          <  1:623755/81‑1 (MQ=255)                                                          gATCGTCGTCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAAcg         <  2:623755/81‑1 (MQ=255)                                                             cgtcgtCATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAACGCgg      >  2:623819/1‑81 (MQ=255)                                                              gtcgtcATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAACGCgg      >  1:623805/1‑80 (MQ=255)                                                                 gtcATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAACGCGGCAt   <  2:623758/80‑1 (MQ=255)                                                                 gtcATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAACGCGGCAt   <  1:623758/80‑1 (MQ=255)                                                                 gtcATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAACGCGGCATg  <  1:623757/81‑1 (MQ=255)                                                                   cATTCAATGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAACGCGGCATg  >  2:623801/1‑79 (MQ=255)                                                                         |                                                                         TATTAGTGCGCTGCATAGCAAACGCGGCGTCGTTCAGCGCCTGCAACCACGGTTTGCGATCGTCGTCATTCAGTGGTTCGCCAGACGCCATTTTTTCGATATTGCGCCGTGGATGGAGGAAATCGCCATCAAGAAACGCGGCATG  >  NC_000913/3577352‑3577496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑