Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	351,307	A→G	K31K (AAA→AAG)	prpD →	2‑methylcitrate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	351,307	0	A	G	100.0%	88.6 / NA	26	K31K (AAA→AAG)	prpD	2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/8);  total (18/8)

AACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCC  >  NC_000913/351230‑351382                                                                              |                                                                            aacaacATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGt                                                                           <  1:72403/80‑1 (MQ=255)  acaacaTCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTgg                                                                         >  1:72450/1‑81 (MQ=255)  acaacaTCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTgg                                                                         >  2:72440/1‑81 (MQ=255)  acaacaTCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTgg                                                                         <  2:72404/81‑1 (MQ=255)   caacaTCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGc                                                                        >  2:72455/1‑81 (MQ=255)              cGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCg                                                             >  1:72447/1‑81 (MQ=255)                  aTTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGcaca                                                         >  1:72435/1‑81 (MQ=255)                    ttGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACAt                                                        >  1:72441/1‑80 (MQ=255)                       aTCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTAct                                                    >  2:72446/1‑81 (MQ=255)                            gAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTActgcctg                                               >  2:72448/1‑81 (MQ=255)                                  gTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGa                                          >  2:72449/1‑80 (MQ=255)                                   ttGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGaca                                        >  2:72442/1‑81 (MQ=255)                                    tGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGacac                                       >  2:72457/1‑81 (MQ=255)                                    tGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGacac                                       >  1:72439/1‑81 (MQ=255)                                       tatCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCt                                    <  2:72405/81‑1 (MQ=255)                                        atCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTc                                   >  2:72456/1‑81 (MQ=255)                                              gATTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGc                             <  2:72406/81‑1 (MQ=255)                                               aTTACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCg                            <  2:72407/81‑1 (MQ=255)                                                ttACGTCATGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCgg                           >  2:72434/1‑81 (MQ=255)                                                        tGAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAg                   >  2:72462/1‑81 (MQ=255)                                                         gAACTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGc                  >  1:72461/1‑81 (MQ=255)                                                         gAACTACGAAATCAGCCCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCCCGGCTGTGGTCTGGAAGc                  >  2:72452/1‑81 (MQ=255)                                                          aaCTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGc                  <  2:72408/80‑1 (MQ=255)                                                           aCTACGAAATCAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGctc                <  2:72409/81‑1 (MQ=255)                                                                     cAGCTCTAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATAccc      >  1:72453/1‑81 (MQ=255)                                                                           tAAGGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGcc  <  2:72410/79‑1 (MQ=255)                                                                              |                                                                            AACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCC  >  NC_000913/351230‑351382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑