Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,586,933	A→T	intergenic (‑110/‑10)	ggt ← / → yhhA	glutathione hydrolase proenzyme/DUF2756 domain‑containing protein YhhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,586,933	0	A	T	100.0%	103.7 / NA	29	intergenic (‑110/‑10)	ggt/yhhA	glutathione hydrolase proenzyme/DUF2756 domain‑containing protein YhhA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/17);  total (12/17)

CTAAGCCTGGTATATAACTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGAGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATA  >  NC_000913/3586857‑3587006                                                                             |                                                                          cTAAGCCTGGTATATAACTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGAt                                                                       <  2:625793/81‑1 (MQ=255)   aaGCCTGGTATATAACTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATAc                                                                     >  1:625829/1‑81 (MQ=255)    aGCCTGGTATATAACTCTGAATTAATCATCGTTTTGTCGGAAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATAc                                                                     <  2:625794/80‑1 (MQ=255)      ccTGGTATATAACTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATAcgc                                                                   >  1:625841/1‑80 (MQ=255)            atataACTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGaaa                                                            >  1:625838/1‑81 (MQ=255)              ataACTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAAc                                                           >  2:625831/1‑80 (MQ=255)               taACTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACg                                                          <  2:625795/80‑1 (MQ=255)                 aCTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACt                                                       <  1:625798/81‑1 (MQ=255)                 aCTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACt                                                       <  1:625797/81‑1 (MQ=255)                  ctctGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACta                                                      >  2:625839/1‑80 (MQ=255)                       aaTTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCt                                                    <  1:625799/78‑1 (MQ=255)                       aaTTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGAt                                                 <  2:625799/81‑1 (MQ=255)                        aTTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGAtt                                                <  2:625800/81‑1 (MQ=255)                           aaTCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCt                                              <  2:625801/80‑1 (MQ=255)                            atcatcGTTTTGTCGGGAAGCGAGTAAACTTGAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTa                                            <  2:625802/81‑1 (MQ=255)                            atcatcGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCtt                                             <  2:625803/80‑1 (MQ=255)                             tcatcGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTAc                                           >  1:625833/1‑81 (MQ=255)                                            gAAGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGtt                            <  1:625805/81‑1 (MQ=255)                                             aaGCGAGTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGttt                           >  2:625858/1‑81 (MQ=255)                                                   gTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGc                     <  1:625806/81‑1 (MQ=255)                                                   gTAAACTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGc                     >  2:625838/1‑81 (MQ=255)                                                      aaCTTAAAGGATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCttt                  <  1:625807/81‑1 (MQ=255)                                                              ggATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGcc          >  1:625843/1‑81 (MQ=255)                                                              ggATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGcc          <  1:625808/81‑1 (MQ=255)                                                               gATATCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGccc         <  2:625808/81‑1 (MQ=255)                                                                  atCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCAt       >  1:625860/1‑80 (MQ=255)                                                                  atCTTCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCAt       >  1:625844/1‑80 (MQ=255)                                                                      tCAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATa  >  2:625845/1‑81 (MQ=255)                                                                       cAGAGGTGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTaa    <  2:625809/78‑1 (MQ=255)                                                                             |                                                                          CTAAGCCTGGTATATAACTCTGAATTAATCATCGTTTTGTCGGGAAGCGAGTAAACTTAAAGGATATCTTCAGAGGAGGATACGCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATA  >  NC_000913/3586857‑3587006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑