| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,595,767 | C→A | A213S (GCG→TCG) | livH ← | branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,595,767 | 0 | C | A | 98.0% | 171.5 / NA | 49 | A213S (GCG→TCG) | livH | branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (24/24); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGC > NC_000913/3595690‑3595842 | ggCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGcc < 1:628182/81‑1 (MQ=255) cATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAAt > 1:628231/1‑81 (MQ=255) aTAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATc < 2:628182/81‑1 (MQ=255) aaaGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATca > 2:628229/1‑80 (MQ=255) aTGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTc < 2:628183/81‑1 (MQ=255) tAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAgc > 1:628237/1‑81 (MQ=255) ggggTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAgcgc < 2:628185/81‑1 (MQ=255) ggggTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAgcgc < 2:628184/81‑1 (MQ=255) gTTGATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAAt > 2:628237/1‑81 (MQ=255) gTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAAt > 1:628235/1‑81 (MQ=255) ttAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAAt > 1:628242/1‑80 (MQ=255) ttAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATc < 1:628187/81‑1 (MQ=255) tAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCa > 2:628256/1‑81 (MQ=255) gACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcg > 1:628256/1‑81 (MQ=255) cGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggt > 2:628253/1‑81 (MQ=255) gCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtc > 1:628250/1‑81 (MQ=255) ccGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtcg < 2:628187/81‑1 (MQ=255) cGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtcgg > 1:628243/1‑81 (MQ=255) cGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtcgg > 1:628239/1‑81 (MQ=255) gTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtcggt > 1:628234/1‑81 (MQ=255) tAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGTGCAATCACCCGGTCGgtg < 1:628189/81‑1 (MQ=255) aaCTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTa < 2:628189/81‑1 (MQ=255) tGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATg > 1:628240/1‑81 (MQ=255) gACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGc > 2:628250/1‑81 (MQ=255) gACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGc > 2:628240/1‑81 (MQ=255) ccGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCa < 1:628191/81‑1 (MQ=255) cGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCaa < 1:628192/81‑1 (MQ=255) gatcagcaCACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAg > 1:628266/4‑81 (MQ=255) atcagcaCACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAg > 1:628262/3‑80 (MQ=255) tcagcaCACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCa < 2:628192/80‑1 (MQ=255) cagcaCACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAg > 1:628247/1‑78 (MQ=255) agcaCACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAg < 1:628195/80‑1 (MQ=255) agcaCACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAg < 2:628193/80‑1 (MQ=255) agcaCACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGa > 1:628264/1‑81 (MQ=255) cacaCCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACt < 2:628196/81‑1 (MQ=255) cacaCCCCCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACt < 2:628195/81‑1 (MQ=255) cacCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCg > 2:628258/1‑81 (MQ=255) cccGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGcc < 1:628198/81‑1 (MQ=255) gCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCAtt < 1:628199/81‑1 (MQ=255) ccACCGCCGCCATCGCCGCGCCAATCACACAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCAttt > 2:628257/1‑81 (MQ=255) cACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCAtttt > 2:628247/1‑81 (MQ=255) aCCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTc < 2:628201/81‑1 (MQ=255) aCCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTc < 2:628200/81‑1 (MQ=255) aCCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTc < 1:628200/81‑1 (MQ=255) ccgccATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGAt < 1:628203/81‑1 (MQ=255) cgccATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATc > 2:628270/1‑81 (MQ=255) ccATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCtt < 2:628203/81‑1 (MQ=255) cATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTc > 2:628259/1‑81 (MQ=255) cATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTc < 1:628205/81‑1 (MQ=255) gccgaGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCcgc < 2:628205/80‑1 (MQ=255) | GGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGC > NC_000913/3595690‑3595842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |