Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,597,808	T→C	intergenic (‑248/‑176)	livK ← / → panZ	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein/PanD maturation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,597,808	0	T	C	100.0%	92.3 / NA	27	intergenic (‑248/‑176)	livK/panZ	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein/PanD maturation factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/13);  total (14/13)

AATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGTAATTATTGCTGTT  >  NC_000913/3597732‑3597883                                                                             |                                                                            aataTTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAAtt                                                                         >  2:628764/1‑81 (MQ=255) aataTTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAAtt                                                                         >  1:628760/1‑81 (MQ=255)           aTTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCAtt                                                               <  2:628735/81‑1 (MQ=255)              tATTGATAGATAAACAGAAAAAAGTGCCTTAGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCa                                                             <  2:628736/80‑1 (MQ=255)                ttGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAgg                                                           <  2:628737/80‑1 (MQ=255)                 tGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAggg                                                          >  2:628766/1‑80 (MQ=255)                  gatagataAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAgggg                                                         <  2:628738/80‑1 (MQ=255)                    tagataAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGAtt                                                      <  2:628739/81‑1 (MQ=255)                       ataAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATg                                                   >  2:628761/1‑81 (MQ=255)                          aaCAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTg                                                >  1:628775/1‑81 (MQ=255)                            cAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGa                                              >  1:628764/1‑81 (MQ=255)                               aaaaaaGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACAt                                           <  2:628740/81‑1 (MQ=255)                                     gTGCCTTTGTCAGCATAAAATAACGGCACAGAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCa                                     <  2:628741/81‑1 (MQ=255)                                             gTCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTaa                             >  1:628768/1‑81 (MQ=255)                                              tCAGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAAt                            <  1:628743/81‑1 (MQ=255)                                                aGCATAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATg                           <  1:628744/80‑1 (MQ=255)                                                    tAAAATAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGtttt                       >  2:628773/1‑80 (MQ=255)                                                     aaaaTAACGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTaa                     >  1:628778/1‑81 (MQ=255)                                                        aTAACGTCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAAttt                  <  1:628745/81‑1 (MQ=255)                                                          aaCGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTg                >  1:628773/1‑81 (MQ=255)                                                          aaCGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTg                >  2:628771/1‑81 (MQ=255)                                                           aCGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTg                <  2:628746/80‑1 (MQ=255)                                                           aCGGCACAAAGGGCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGt               <  1:628746/81‑1 (MQ=255)                                                                     gggCGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGTAATTATTGCt     <  1:628748/81‑1 (MQ=255)                                                                        cGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGTAATTATTGCTGt   >  1:628780/1‑80 (MQ=255)                                                                        cGGAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGTAATTATTGCTGt   >  2:628769/1‑80 (MQ=255)                                                                         ggAACAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGTAATTATTGCTGtt  >  1:628769/1‑80 (MQ=255)                                                                             |                                                                            AATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGTAATTATTGCTGTT  >  NC_000913/3597732‑3597883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑