Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,611,584	A→G	F50F (TTT→TTC)	yhhS ←	putative transporter YhhS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,611,584	0	A	G	100.0%	128.4 / NA	37	F50F (TTT→TTC)	yhhS	putative transporter YhhS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/22);  total (15/22)

TAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAG  >  NC_000913/3611507‑3611656                                                                              |                                                                         tAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAg                                                                       <  1:632313/81‑1 (MQ=255)     gTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCca                                                                   <  2:632313/81‑1 (MQ=255)      tCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCcat                                                                  >  1:632376/1‑81 (MQ=255)       ccGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCcat                                                                  >  1:632382/1‑80 (MQ=255)         ggCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCcatcac                                                               <  1:632315/81‑1 (MQ=255)            aTGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCGGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCcatcacat                                                             >  2:632389/1‑80 (MQ=255)                 ggcggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGa                                                       >  1:632384/1‑81 (MQ=255)                  gcggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGa                                                       >  2:632366/1‑80 (MQ=255)                            aaGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCgg                                             >  2:632365/1‑80 (MQ=255)                            aaGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGt                                            <  1:632316/81‑1 (MQ=255)                             aGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGt                                            >  2:632367/1‑80 (MQ=255)                             aGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGt                                            >  2:632386/1‑80 (MQ=255)                             aGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTa                                           <  1:632317/81‑1 (MQ=255)                                     aaaTATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACagcg                                   <  2:632317/81‑1 (MQ=255)                                      aaTATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACagcga                                  <  1:632319/81‑1 (MQ=255)                                        tatTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACagcgagc                                <  2:632319/81‑1 (MQ=255)                                         atTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACagcgagcg                               >  1:632392/1‑81 (MQ=255)                                          ttGCAGGCTGATAACCAAGCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACagcgagcg                               <  1:632321/80‑1 (MQ=255)                                             cAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCaa                           <  1:632323/81‑1 (MQ=255)                                             cAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCa                            <  1:632322/80‑1 (MQ=255)                                               ggCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAAcc                         <  1:632324/81‑1 (MQ=255)                                                 cTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCg                       <  2:632324/81‑1 (MQ=255)                                                      aaCCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGt                  >  1:632388/1‑81 (MQ=255)                                                       aCCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGt                  <  2:632325/80‑1 (MQ=255)                                                         cAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAg               <  1:632327/81‑1 (MQ=255)                                                           aTCCTGCCCAGAAGGCGCTGAAGCCCATCGCATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGt             <  1:632329/81‑1 (MQ=255)                                                           aTCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGt             <  2:632327/81‑1 (MQ=255)                                                            tCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTa            <  1:632330/81‑1 (MQ=255)                                                             ccTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAg           >  2:632376/1‑81 (MQ=255)                                                              cTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGc          <  2:632330/81‑1 (MQ=255)                                                               tGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCt         >  1:632399/1‑81 (MQ=255)                                                                 cccAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTgg       <  2:632331/81‑1 (MQ=255)                                                                  ccAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGc      <  2:632332/81‑1 (MQ=255)                                                                   cAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGc      >  1:632373/1‑80 (MQ=255)                                                                     gAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCg     >  2:632384/1‑79 (MQ=255)                                                                      aaGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAg  <  2:632333/81‑1 (MQ=255)                                                                      aaGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAg  >  2:632400/1‑81 (MQ=255)                                                                              |                                                                         TAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAG  >  NC_000913/3611507‑3611656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑